Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
Angew Chem Int Ed Engl. 2021 May 17;60(21):11580-11591. doi: 10.1002/anie.202010278. Epub 2021 Jan 26.
The cell membrane is not only a physical barrier, but also a functional organelle that regulates the communication between a cell and its environment. The ability to functionalize the cell membrane with synthetic molecules or nanostructures would advance cellular functions beyond what evolution has provided. The aim of this Minireview is to introduce recent progress in using synthetic DNA and DNA-based nanostructures for cell-surface engineering. We first introduce chemical conjugation and physical binding methods for monovalent and polyvalent surface engineering. We then introduce the application of these methods for either the promotion or inhibition of cell-environment communication in numerous applications, including the promotion of cell-cell recognition, regulation of intracellular pathways, protection of therapeutic cells, and sensing of the intracellular and extracellular microenvironments. Lastly, we summarize current challenges existing in this area and potential solutions to solve these challenges.
细胞膜不仅是物理屏障，还是调节细胞与其环境之间通讯的功能细胞器。用合成分子或纳米结构对细胞膜进行功能化修饰，可以使细胞功能超越进化所赋予的范围。本综述旨在介绍利用合成 DNA 和基于 DNA 的纳米结构进行细胞表面工程的最新进展。我们首先介绍单价和多价表面工程的化学偶联和物理结合方法。然后介绍这些方法在众多应用中促进或抑制细胞-环境通讯的应用，包括促进细胞间识别、调节细胞内途径、保护治疗细胞以及感测细胞内和细胞外微环境。最后，我们总结了该领域目前存在的挑战和潜在的解决方案。
