Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, United States.
J Am Soc Mass Spectrom. 2022 Feb 2;33(2):296-303. doi: 10.1021/jasms.1c00300. Epub 2022 Jan 21.
Mass spectrometry imaging provides a powerful approach for the direct analysis and spatial visualization of molecules in tissue sections. Using matrix-assisted laser desorption/ionization mass spectrometry, intact protein imaging has been widely investigated for biomarker analysis and diagnosis in a variety of tissue types and diseases. However, blood-rich or highly vascular tissues present a challenge in molecular imaging due to the high ionization efficiency of hemoglobin, which leads to ion suppression of endogenous proteins. Here, we describe a protocol to selectively reduce hemoglobin signal in blood-rich tissues that can easily be integrated into mass spectrometry imaging workflows.
质谱成像为直接分析和可视化组织切片中的分子提供了强大的方法。使用基质辅助激光解吸/电离质谱，完整蛋白质成像已广泛应用于各种组织类型和疾病的生物标志物分析和诊断。然而，富含血液或高度血管化的组织由于血红蛋白的高离子化效率，给内源性蛋白质的离子抑制带来了挑战。在这里，我们描述了一种选择性降低富含血液组织中血红蛋白信号的方案，该方案可轻松集成到质谱成像工作流程中。
