Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, 11566, Cairo, Egypt.
Department of Drug Radiation Research, National Center for Radiation Research & Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
Biomed Pharmacother. 2022 Feb;146:112603. doi: 10.1016/j.biopha.2021.112603. Epub 2021 Dec 29.
Radiation enteritis is the most common complication of radiotherapy in patients with pelvic malignancies. Thus, the radioprotective activity of the total hydro-alcoholic extract (BGE) and the ethyl acetate soluble fraction (EAF) of Brownea grandiceps leaves was evaluated against ϒ-radiation-induced enteritis in rats. (BGE) and (EAF) were characterized using HPLC-PDA-ESI-MS/MS analysis. The total phenolic and flavonoid contents were also quantified. In vivo administration of (BGE) (400 mg/kg) and (EAF) (200 & 400 mg/kg) prevented intestinal injury and maintained the mucosal integrity of irradiated rats through increasing villi length and promoting crypt regeneration. Also, (EAF) showed more potent antioxidant activity than (BGE) through reduction of MDA level and enhancement of GSH content and catalase enzyme activity. (BGE) and (EAF) down-regulated intestinal NF-κB expression leading to diminished expression of downstream inflammatory cytokine TNF-α. Moreover, (EAF) markedly reduced the expression of profibrotic marker TGF-β1. Seventy-nine compounds were tentatively identified, including flavonoids, proanthocyanidins, polar lipids and phenolic acids. (EAF) showed significantly higher total phenolic and flavonoid contents, as compared to (BGE). Results revealed remarkable radioprotective activity of (BGE) and (EAF), with significantly higher activity for (EAF). The chemical constituents of (BGE) and (EAF) strongly supported their radioprotective activity. To the best of our knowledge, the present study describes for the first time the radioprotective activity of B. grandiceps leaves in relation to its secondary metabolome fingerprint; emphasizing the great promise of B. grandiceps leaves, especially (EAF), to be used as natural radio-protective agent.
放射性肠炎是盆腔恶性肿瘤患者放疗最常见的并发症。因此，评估了 Brownea grandiceps 叶的全水-醇提取物 (BGE) 和乙酸乙酯可溶部分 (EAF) 对大鼠 γ 射线诱导肠炎的放射保护活性。(BGE) 和 (EAF) 采用 HPLC-PDA-ESI-MS/MS 分析进行了表征。还定量了总酚和类黄酮的含量。体内给予 (BGE) (400 mg/kg) 和 (EAF) (200 和 400 mg/kg) 可通过增加绒毛长度和促进隐窝再生来预防肠损伤并维持受照大鼠的黏膜完整性。此外，(EAF) 通过降低 MDA 水平、增强 GSH 含量和过氧化氢酶活性，表现出比 (BGE) 更强的抗氧化活性。(BGE) 和 (EAF) 下调肠道 NF-κB 表达，导致下游炎症细胞因子 TNF-α表达减少。此外，(EAF) 显著降低了促纤维化标志物 TGF-β1 的表达。共推测出 79 种化合物，包括类黄酮、原花青素、极性脂质和酚酸。(EAF) 的总酚和类黄酮含量明显高于 (BGE)。结果表明，(BGE) 和 (EAF) 具有显著的放射保护活性，(EAF) 的活性明显更高。BGE 和 EAF 的化学成分强烈支持其放射保护活性。据我们所知，本研究首次描述了 B. grandiceps 叶与次生代谢物指纹图谱相关的放射保护活性；强调了 B. grandiceps 叶，尤其是 (EAF)，具有作为天然放射保护剂的巨大潜力。
