低浓度黄曲霉毒素 B1 污染日粮中添加吸附剂对肉鸡亚临床症状和组织病变的预防作用及机制。
The preventive effect and mechanisms of adsorbent supplementation in low concentration aflatoxin B1 contaminated diet on subclinical symptom and histological lesions of broilers.
机构信息
College of Animal Science and Technology, Hunan Engineering Research Center for Poultry Safety, Hunan Agricultural University, Changsha 410128, Hunan, China; Course of Biological Science and Technology, United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
College of Animal Science and Technology, Hunan Engineering Research Center for Poultry Safety, Hunan Agricultural University, Changsha 410128, Hunan, China; Hunan Co-Innovation Center of Animal Production Safety, Engineering Research Center, Changsha 410128, Hunan, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, Hunan, China.
出版信息
Poult Sci. 2022 Mar;101(3):101634. doi: 10.1016/j.psj.2021.101634. Epub 2021 Dec 8.
This study aimed to investigate the subclinical symptom and histological lesions of 21-day-old and 42-day-old broilers exposure to low concentration aflatoxin B1 (AFB), and the preventive effect with adsorbent (Toxo-MX) supplementation. A total of 576 one-day-old Arbor Acres broilers were randomly allotted into 6 treatments 8 replicates and 12 birds per cage, fed with 0 ppb, 60 ppb and 120 ppb AFB contamination diet with or without Toxo-MX supplementation. Results showed both 60 ppb and 120 ppb AFB contamination significantly reduced growth performance in 21-day-old broilers (P < 0.05), but not in 42-day-old broilers (P > 0.05), however, AFB contamination in diet caused a higher feed to gain ratio (P < 0.05). Broilers of 21-day-old exposure to 60 ppb and 120 ppb AFB increased mRNA expression of hepatic inflammatory cytokines, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity (P < 0.05), 42-day-old broilers showed a same change in 120 ppb but not in 60 ppb of AFB contamination (P < 0.05). mRNA expressions of clauding-1, Zonula occludens-1 (ZO-1), and occludin decreased, but Bax, Bcl-2, and caspase-3 increased in 21-day-old broilers exposure to 60 ppb and 120 ppb AFB (P < 0.05), broilers of 42-day-old resisted on intestinal aflatoxicosis impairment against 60 ppb AFB contamination (P < 0.05), but not in 120 ppb (P < 0.05). Toxo-MX supplementation significantly reversed the detrimental effects on growth performance in both age broilers and reduced the accelerated feed to gain ratio caused by AFB (P < 0.05). Intestinal mRNA expression of tight junction and apoptotic genes in both age broilers were recovered by Toxo-MX supplementation (P < 0.05). However, Toxo-MX did not restore the accelerated expression of hepatic inflammation cytokines and SOD, GSH-Px in 120ppb AFB group (P < 0.05). The data demonstrated that diet supplementation with Toxo-MX reversed the detrimental effect on growth performance and intestine in broilers exposure to 60 ppb and 120 ppb AFB1. However, did not completely recovered hepatic inflammation induced by AFB
本研究旨在探讨 21 日龄和 42 日龄肉鸡暴露于低浓度黄曲霉毒素 B1（AFB）时的亚临床症状和组织学病变，以及吸附剂（Toxo-MX）补充剂的预防作用。总共 576 只 1 日龄的阿伯丁肉鸡被随机分为 6 个处理组，每组 8 个重复，每个笼子 12 只鸡，分别饲喂 0 ppb、60 ppb 和 120 ppb 的 AFB 污染日粮，或添加或不添加 Toxo-MX。结果表明，60 ppb 和 120 ppb 的 AFB 污染均显著降低了 21 日龄肉鸡的生长性能（P < 0.05），但在 42 日龄肉鸡中没有（P > 0.05），然而，日粮中的 AFB 污染导致更高的饲料增重比（P < 0.05）。暴露于 60 ppb 和 120 ppb AFB 的 21 日龄肉鸡的肝脏炎性细胞因子和超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性的 mRNA 表达增加（P < 0.05），42 日龄肉鸡在 120 ppb 的 AFB 污染下表现出相同的变化，但在 60 ppb 时没有（P < 0.05）。Clauding-1、Zonula occludens-1（ZO-1）和闭合蛋白的 mRNA 表达降低，但 Bax、Bcl-2 和 caspase-3 在暴露于 60 ppb 和 120 ppb AFB 的 21 日龄肉鸡中增加（P < 0.05），42 日龄肉鸡抵抗 60 ppb AFB 污染对肠道黄曲霉毒素损害（P < 0.05），但对 120 ppb 没有（P < 0.05）。Toxo-MX 补充剂显著逆转了对两个年龄段肉鸡生长性能的不利影响，并降低了 AFB 引起的加速饲料增重比（P < 0.05）。Toxo-MX 补充剂恢复了两个年龄段肉鸡肠道紧密连接和凋亡基因的 mRNA 表达（P < 0.05）。然而，Toxo-MX 并没有恢复 120ppb AFB 组中肝脏炎症细胞因子和 SOD、GSH-Px 的加速表达（P < 0.05）。数据表明，日粮补充 Toxo-MX 逆转了 60 ppb 和 120 ppb AFB1 暴露对肉鸡生长性能和肠道的不利影响。然而，它并没有完全恢复 AFB 引起的肝脏炎症。
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