Lala A O, Ajayi O L, Oso A O, Ajao M O, Oni O O, Okwelum N, Idowu O M O
Institute of Food Security, Environmental Resources and Agricultural Research, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Department of Veterinary Pathology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
J Anim Physiol Anim Nutr (Berl). 2016 Dec;100(6):1191-1202. doi: 10.1111/jpn.12421. Epub 2015 Dec 14.
This study was carried out to investigate the effect of dietary supplementation with molecular or nano-clay binders on biochemical and histopathological examination of organs of turkeys fed diets contaminated with aflatoxin B Two hundred and sixteen unsexed 1-day-old British United Turkeys were randomly allotted to nine diets in a 3 × 3 factorial arrangement of diets supplemented with no toxin binder, molecular toxin binder (MTB) and nano-clay toxin binder, each contaminated with 0, 60 and 110 ppb aflatoxin B respectively. There were three replicates per treatment with eight turkeys per replicate. Biochemical analyses, organ weights and histopathological changes of some organs were examined at the end of the study which lasted for 84 days. Turkeys fed diets supplemented with molecular and nano-binders showed higher (p < 0.001) total serum protein, reduced (p < 0.001) serum uric acid and GGT concentration values when compared with those fed aflatoxin-contaminated diets supplemented with no binder. Turkeys fed aflatoxin-contaminated diets supplemented with no binder had increased (p < 0.001) AST and ALT concentration when compared with other treatments. The heaviest (p < 0.001) liver and intestinal weight was noticed with turkeys fed diets supplemented with no binder and contaminated with 110 ppb aflatoxin B . Pathologically, there was no visible morphological alteration noticed in all turkeys fed uncontaminated diets and nano-clay-supplemented group. Hepatic paleness, hepatomegaly and yellowish discolouration of the liver were observed with turkeys fed diets containing no binder but contaminated with 60 and 110 ppb aflatoxin B1. Intestinal histopathological changes such as goblet cell hyperplasia, villous atrophy and diffuse lymphocytic enteritis were more prominent in turkeys fed diets containing no toxin binder and MTB. In conclusion, there were improved biochemical parameters and reduced deleterious effects of aflatoxin B in turkeys fed diet supplemented with clay binders. However, the improvement was more conspicuous in the nano-clay-supplemented group than molecular clay group.
本研究旨在探讨日粮中添加分子或纳米粘土结合剂对饲喂受黄曲霉毒素B污染日粮的火鸡器官生化及组织病理学检查的影响。将216只1日龄未分性别的英国联合火鸡随机分为9组日粮，采用3×3析因设计，分别添加无毒素结合剂、分子毒素结合剂（MTB）和纳米粘土毒素结合剂，每组日粮分别污染0、60和110 ppb黄曲霉毒素B。每个处理设3个重复，每个重复8只火鸡。在为期84天的研究结束时，对一些器官进行了生化分析、器官重量测定和组织病理学变化检查。与饲喂未添加结合剂的黄曲霉毒素污染日粮的火鸡相比，饲喂添加分子和纳米结合剂日粮的火鸡血清总蛋白含量更高（p<0.001），血清尿酸和γ-谷氨酰转移酶（GGT）浓度值降低（p<0.001）。与其他处理相比，饲喂未添加结合剂的黄曲霉毒素污染日粮的火鸡天门冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）浓度升高（p<0.001）。饲喂未添加结合剂且污染110 ppb黄曲霉毒素B日粮的火鸡肝脏和肠道重量最重（p<0.001）。病理上，在所有饲喂未污染日粮和纳米粘土添加组的火鸡中未观察到明显的形态学改变。在饲喂未添加结合剂但污染60和110 ppb黄曲霉毒素B1日粮的火鸡中，观察到肝脏苍白、肝肿大和肝脏发黄。在饲喂未添加毒素结合剂和MTB日粮的火鸡中，肠道组织病理学变化如杯状细胞增生、绒毛萎缩和弥漫性淋巴细胞性肠炎更为明显。总之，在饲喂添加粘土结合剂日粮的火鸡中，生化参数得到改善，黄曲霉毒素B的有害影响降低。然而，纳米粘土添加组的改善比分子粘土组更明显。
