State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu, 214122, PR China.
NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing, 100021, PR China.
Biosens Bioelectron. 2022 Apr 15;202:113990. doi: 10.1016/j.bios.2022.113990. Epub 2022 Jan 13.
The rapid, efficient, and objective evaluation of active antioxidant components is of great significance for the basic research of natural products and food quality. In this work, a three-dimensional (3D) cell culture system-based electrochemical biosensor for HO detection based on the relationship between the HO extracellular level and the current signal response was developed, which could be used for evaluating the antioxidant activity of active compounds. To increase the analytical selectivity and the response specificity, an A549 cells/hydrogel@carbon nanofibers (CNFs)/manganese dioxide nanowires (MnONWs)/gold nanoparticles (AuNPs)-modified electrochemical biosensor was successfully prepared based on the catalytic reaction between the response of HO and MnO to the current signal. Under the optimized modification parameters of the working electrode surface, a good linear correlation was found between the oxidation peak current (Ip) value and the HO concentration induced by paraquat. The linear equation was Ip(μA) = 58.199C+5.825 (C for HO concentration) with R = 0.993, and the detection limit of HO was 0.02 μM, which indicated high sensitivity, satisfactory reproducibility, and stability of this method. The biosensor was successfully used to evaluate and grade the antioxidant activity of 16 anthocyanins and their glycosidic derivatives, indicating the feasibility of this method for the antioxidant evaluation of natural products. This proposed method provides a new way for evaluating the in vitro efficacy of natural products based on their physiological activities and for designing a new sensing platform.
快速、高效、客观地评价活性抗氧化成分对于天然产物和食品质量的基础研究具有重要意义。在这项工作中，基于 HO 细胞外水平与电流信号响应之间的关系，开发了一种用于检测 HO 的基于三维（3D）细胞培养体系的电化学生物传感器，可用于评估活性化合物的抗氧化活性。为了提高分析选择性和响应特异性，基于 HO 与 MnO 对电流信号的催化反应，成功制备了基于 A549 细胞/水凝胶@碳纳米纤维（CNFs）/二氧化锰纳米线（MnONWs）/金纳米粒子（AuNPs）修饰的电化学生物传感器。在工作电极表面的最佳修饰参数下，发现百草枯诱导的 HO 引起的氧化峰电流（Ip）值与 HO 浓度之间存在良好的线性相关性。线性方程为 Ip（μA）= 58.199C+5.825（C 为 HO 浓度），R = 0.993，HO 的检测限为 0.02 μM，表明该方法具有高灵敏度、令人满意的重现性和稳定性。该生物传感器成功用于评估和分级 16 种花色苷及其糖苷衍生物的抗氧化活性，表明该方法用于评估天然产物的抗氧化活性是可行的。该方法为基于生理活性评估天然产物的体外功效和设计新型传感平台提供了一种新方法。
