Chen Ding-Kang, Shao Hui-Yan, Yang Liu, Hu Jiang-Miao
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
R&D Center of Dr. Plant, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Nat Prod Bioprospect. 2022 Jan 27;12(1):1. doi: 10.1007/s13659-022-00323-6.
Dendrobium officinale is a valuable medicinal herb that is widely used in traditional Chinese medicine. The chemical constituents of D. officinale have attracted much attention and a large number of compounds have been reported including many bibenzyl derivatives. 13 bibenzyl derivatives from D. officinale were sent for molecular docking, surface plasmon resonance (SPR) assay and after detection of Mn-SOD and SIRT3 activities in or not in HaCaT cells, it was concluded that bibenzyl derivatives did not directly activate Mn-SOD but promoted SIRT3 proteins. In addition, HaCaT cells were irradiated with UV-B to induce an oxidative stress model in vitro to further verify the effect of bibenzyl derivatives. The results show that bibenzyl derivatives could directly bind to SIRT3, enhance the deacetylation and then activate Mn-SOD, so as to protect UV-B induced skin photoaging.
铁皮石斛是一种珍贵的草药，在传统中药中广泛应用。铁皮石斛的化学成分备受关注，已报道了大量化合物，包括许多联苄衍生物。从铁皮石斛中提取的13种联苄衍生物被送去进行分子对接、表面等离子体共振（SPR）分析，并检测其在HaCaT细胞中是否具有锰超氧化物歧化酶（Mn-SOD）和沉默信息调节因子3（SIRT3）活性，结果得出联苄衍生物不会直接激活Mn-SOD，但会促进SIRT3蛋白的生成。此外，用UV-B照射HaCaT细胞以在体外诱导氧化应激模型，进一步验证联苄衍生物的作用效果。结果表明，联苄衍生物可直接与SIRT3结合，增强去乙酰化作用，进而激活Mn-SOD，从而保护UV-B诱导的皮肤光老化。
