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长喙壳菌/拟茎点霉通过分泌漆酶来降解一系列双酚类似物。

Diaporthe/Phomopsis longicolla degrades an array of bisphenol analogues with secreted laccase.

机构信息

Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines.

Institute of Chemistry, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines.

出版信息

Microbiol Res. 2022 Apr;257:126973. doi: 10.1016/j.micres.2022.126973. Epub 2022 Jan 19.

Abstract

With recent initiatives to ban bisphenol A (BPA) in certain commercial products, manufacturers shifted to the production and use of BPA analogues. However, some of these BPA alternatives still possess endocrine disruptive activities. Many fungal enzymes are known to biodegrade phenolic compounds, such as BPA. However, the activity of these enzymes on BPA analogues remains unexplored. This study reports a secreted laccase from the endophytic fungus Diaporthe longicolla capable of degrading an impressive range of bisphenol analogues. The secreted crude enzymes are optimally active at pH 5 from 39 °C to 60 °C, efficiently degrading BPA as well as BPA analogues BPB, BPC, BPE and BPF. A purified form of laccase was identified from the crude fungal extract using FPLC and peptide sequencing. Furthermore, BPA induced the expression of this D. longicolla laccase gene. Overall, this paper demonstrated that the crude laccase enzyme from D. longicolla metabolizes BPA and select analogues, implicating the potential role of this fungus to remove environmental bisphenols.

摘要

随着最近禁止某些商业产品中使用双酚 A(BPA)的举措,制造商转向生产和使用 BPA 类似物。然而,其中一些 BPA 替代品仍具有内分泌干扰活性。许多真菌酶已知能够生物降解酚类化合物,如 BPA。然而,这些酶对 BPA 类似物的活性仍未得到探索。本研究报道了一种来自内生真菌 Diaporthe longicolla 的分泌漆酶,能够降解一系列令人印象深刻的双酚类似物。分泌的粗酶在 pH5 下从 39°C 到 60°C 时具有最佳活性,能够有效降解 BPA 以及 BPA 类似物 BPB、BPC、BPE 和 BPF。使用 FPLC 和肽测序从粗真菌提取物中鉴定出一种纯化形式的漆酶。此外,BPA 诱导了这种 D. longicolla 漆酶基因的表达。总的来说,本文证明了 D. longicolla 的粗漆酶能够代谢 BPA 和选择的类似物,这暗示了这种真菌在去除环境双酚方面的潜在作用。

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