一种从体外构建的杂交λ溶原菌中大规模纯化DNA连接酶的简单三步程序。
A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lambda lysogen constructed in vitro.
作者信息
Panasenko S M, Alazard R J, Lehman I R
出版信息
J Biol Chem. 1978 Jul 10;253(13):4590-2.
PMID:350879
Abstract
A simple three-step procedure for the large scale purification of DNA ligase has been developed. THe source of enzyme is a strain of Escherichia coli with a hybrid lambda prophage constructed in vitro that bears the ligase overproducing gene lop 11 lig+ (Panasenko, S., Cameron, J., Davis, R. W., and Lehman, I. R. (1977) Science 196, 188-189). The procedure yields homogeneous enzyme in approximately 40% yield.
摘要
已开发出一种用于大规模纯化DNA连接酶的简单三步程序。酶的来源是一种带有体外构建的杂交λ原噬菌体的大肠杆菌菌株，该噬菌体携带连接酶高产基因lop 11 lig +（帕纳先科，S.，卡梅伦，J.，戴维斯，R. W.，和莱曼，I. R.（1977年）《科学》196，188 - 189）。该程序可产生约40%产率的纯酶。
Zotero 插件