Ishino Y, Shinagawa H, Makino K, Tsunasawa S, Sakiyama F, Nakata A
Mol Gen Genet. 1986 Jul;204(1):1-7. doi: 10.1007/BF00330179.
The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.
大肠杆菌的DNA连接酶催化DNA中5'-磷酸基和3'-羟基之间依赖烟酰胺腺嘌呤二核苷酸（NAD）形成磷酸二酯键。它对于DNA复制和受损DNA链的修复至关重要。我们测定了大肠杆菌中编码DNA连接酶及其侧翼区域的lig基因的核苷酸序列。通过纯化的连接酶的氨基酸组成以及氨基末端和羧基末端氨基酸序列证实了该基因的编码框架。该连接酶由671个氨基酸残基组成，分子量为73,690。
