枣椰树茎皮提取物对人癌细胞系的体外细胞毒性分析。
In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines.
作者信息
Rajendrasozhan Saravanan, Ads Essam N, Abouzied Amr S, Humaidi Jamal R
机构信息
Department of Chemistry, College of Sciences, University of Haail, Haail 55476, Kingdom of Saudi Arabia.
Faculty of Science, Zagazig University, El Mansoura 35522, Egypt.
出版信息
Bioinformation. 2021 May 31;17(5):583-592. doi: 10.6026/97320630017583. eCollection 2020.
Zizyphus spina-christi (Rhamnaceae family) is an edible plant used in folk medicine. Therefore, it is of interest to report the cytotoxic effects of Z. spina-christi bark crude extract on human cell lines. Crude ethanol extract of Z. spina-christi bark was fractionated with increasing polarity (diethyl ether, chloroform, ethyl acetate and butanol fractions). The fractions were examined for their cytotoxicity against human colon cancer (HCT-116 and CACO-2), cervical cancer (HeLa and HEp-2), lung carcinoma (A-549), hepatocellular carcinoma (HepG-2), breast cancer (MCF-7) and prostate cancer (PC-3) cell lines using viability assay. Diethyl ether fraction of Z. spina-christi showed the highest cytotoxic effects among the four extracts of Z. spina-christi. The IC50 of diethyl ether fraction was 7.14, 11.2, 11.6, 15.4, 39.8, 42.2, 84.2 and 153.8 µg/ml on HepG-2, A-549, CACO-2, HCT-116, MCF-7, PC-3, HeLa, and HEp-2 cell lines, respectively. Data shows that the diethyl ether fraction of Z. spina-christi showed effective cytotoxic effects in colon, lung and hepatocellular cancer cell lines.
枣（鼠李科）是一种可食用植物，在民间医学中有所应用。因此，报告枣树皮粗提物对人类细胞系的细胞毒性作用具有重要意义。枣树皮的粗乙醇提取物按照极性递增的顺序进行了分离（得到乙醚、氯仿、乙酸乙酯和丁醇馏分）。使用活力测定法检测了这些馏分对人结肠癌（HCT - 116和CACO - 2）、宫颈癌（HeLa和HEp - 2）、肺癌（A - 549）、肝细胞癌（HepG - 2）、乳腺癌（MCF - 7）和前列腺癌（PC - 3）细胞系的细胞毒性。枣的乙醚馏分在枣的四种提取物中显示出最高的细胞毒性作用。乙醚馏分对HepG - 2、A - 549、CACO - 2、HCT - 116、MCF - 7、PC - 3、HeLa和HEp - 2细胞系的IC50分别为7.14、11.2、11.6、15.4、39.8、42.2、84.2和153.8μg/ml。数据表明，枣的乙醚馏分在结肠癌、肺癌和肝细胞癌细胞系中显示出有效的细胞毒性作用。
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