Matsui T, Takano M, Miyamoto K, Itoh Y, Yoshizawa H, Koike M, Mochizuki T, Tanaka E, Okamoto H, Imai M
Gastroenterology. 1986 Jan;90(1):135-42. doi: 10.1016/0016-5085(86)90085-5.
A primary hepatocellular carcinoma, from a patient carrying hepatitis B virus, was transplanted to athymic nude mice, and maintained through eight passages involving 39 mice. Hepatitis B surface and e antigens were detected in the circulation of tumor-bearing mice. Hepatitis B surface, core, and e antigens were demonstrated in tumor cells. Hepatitis B core particles were visualized in the tumor extract by immune electron microscopy, and they exhibited an activity of deoxyribonucleic acid polymerase. In the tumor tissue, deoxyribonucleic acid of hepatitis B virus was present both in integrated and extrachromosomal forms. Nude mice carrying the tumor would provide opportunities for studying the replication of hepatitis B virus and the expression of its various proteins, and also for evaluating the efficacy of virustatic drugs in interrupting the persistent infection.
将一名携带乙肝病毒患者的原发性肝细胞癌移植到无胸腺裸鼠体内，并通过涉及39只小鼠的八代传代得以维持。在荷瘤小鼠的循环系统中检测到乙肝表面抗原和e抗原。在肿瘤细胞中证实存在乙肝表面抗原、核心抗原和e抗原。通过免疫电子显微镜在肿瘤提取物中观察到乙肝核心颗粒，并且它们表现出脱氧核糖核酸聚合酶活性。在肿瘤组织中，乙肝病毒的脱氧核糖核酸以整合形式和染色体外形式存在。携带该肿瘤的裸鼠将为研究乙肝病毒的复制及其各种蛋白质的表达提供机会，也为评估抗病毒药物在阻断持续感染方面的疗效提供机会。
