Department of General Medicine, The First People's Hospital of Shangqiu City, Shangqiu, Henan, China.
Department of Joint Surgery, The First People's Hospital of Shangqiu City, Shangqiu, Henan, China.
Bioengineered. 2022 Jan;13(1):469-480. doi: 10.1080/21655979.2021.2009414.
Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude mice to observe tumor growth . The interaction circ_0000654, miR-149-5p, and INHBA was validated. circ_0000654 and INHBA were up-regulated but miR-149-5p was down-regulated in GC. circ_0000654 absorbed miR-149-5p to target INHBA. Silencing circ_0000654inhibited the progress of GC cell biology. Oppositely, restoring circ_0000654 enhanced the growth of GC cells. Inhibiting miR-149-5p rescued down-regulated circ_0000654-induced anti-tumor effect on GC. circ_0000654 silence or miR-149-5p overexpression limited the growth of GC tumors . Obviously, circ_0000654 facilitates the growth of GC cells through absorbing miR-149-5p to up-regulate INHBA.
环状 RNA（circRNA）在胃癌（GC）中差异表达，并参与肿瘤细胞的生物生长。鉴于此，进行了研究以揭示 circ_0000654 在 GC 中的功能。获得 GC 组织和正常组织标本，检测 circ_0000654、microRNA（miR）-149-5p 和抑制素-βA（INHBA）水平。转染 GC 细胞系（BGC-823）以改变 circ_0000654 和 miR-149-5p 的表达，从而观察细胞恶性程度。将稳定转染的 BGC-823 细胞注射到裸鼠中观察肿瘤生长。验证 circ_0000654、miR-149-5p 和 INHBA 的相互作用。GC 中 circ_0000654 和 INHBA 上调，但 miR-149-5p 下调。circ_0000654 吸收 miR-149-5p 以靶向 INHBA。沉默 circ_0000654 抑制 GC 细胞生物学的进展。相反，恢复 circ_0000654 增强了 GC 细胞的生长。抑制 miR-149-5p 挽救了下调的 circ_0000654 对 GC 的抗肿瘤作用。circ_0000654 沉默或 miR-149-5p 过表达限制了 GC 肿瘤的生长。显然，circ_0000654 通过吸收 miR-149-5p 来上调 INHBA 促进 GC 细胞的生长。
