Yao Da, Lin Shengcheng, Chen Size, Wang Zhe
Department of Thoracic Surgery, Shenzhen Second People's Hospital. The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.
Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and PeKing Union Medical College, Shenzhen, Guangdong, China.
Bioengineered. 2022 Apr;13(4):9767-9780. doi: 10.1080/21655979.2022.2060776.
Circular RNAs (circRNAs) are a type of important non-coding RNAs that widely involve in the physiological and pathophysiological process. Recent research has established a link between circHIPK3 and the malignant activity of cancer cells. However, circHIPK3' role in esophageal squamous cell carcinoma (ESCC) still needs more focus. To determine the prognostic value of circHIPK3 in patients with ESCC, the expression of circHIPK3 was quantified in 32 pairs of ESCC using real-time polymerase chain reaction (RT-qPCR). Then, the correlation between circHIPK3 expression and clinical characteristics of patients was also analyzed. The function of circHIPK3 in the development of ESCC was investigated using cell biology studies and bioinformatics. The results showed that the expression of circHIPK3 was considerably higher in tumor tissues from ESCC patients than that of adjacent tissues, which was associated with a poor prognosis. Additionally, silencing of circHIPK3 expression retarded esophageal cancer cell proliferation, migration and epithelial-mesenchymal transition (EMT) , as well as the growth . Mechanistically, we discovered that circHIPK3 behaved like a sponge, absorbing microRNA-124 (miR-124) and promoting serine/threonine kinase 3 () expression. Our findings indicate that circHIPK3 acts as an oncogene in ESCC and that the circHIPK3- axis may be a therapeutic target for patients with ESCC.
环状RNA（circRNAs）是一类重要的非编码RNA，广泛参与生理和病理生理过程。最近的研究已经建立了circHIPK3与癌细胞恶性活性之间的联系。然而，circHIPK3在食管鳞状细胞癌（ESCC）中的作用仍需要更多关注。为了确定circHIPK3在ESCC患者中的预后价值，使用实时聚合酶链反应（RT-qPCR）对32对ESCC组织中circHIPK3的表达进行了定量分析。然后，还分析了circHIPK3表达与患者临床特征之间的相关性。利用细胞生物学研究和生物信息学方法研究了circHIPK3在ESCC发生发展中的作用。结果显示，ESCC患者肿瘤组织中circHIPK3的表达明显高于癌旁组织，且与预后不良相关。此外，circHIPK3表达的沉默抑制了食管癌细胞的增殖、迁移和上皮-间质转化（EMT）以及生长。机制上，我们发现circHIPK3起到海绵作用，吸附微小RNA-124（miR-124）并促进丝氨酸/苏氨酸激酶3（）的表达。我们的研究结果表明，circHIPK3在ESCC中作为癌基因发挥作用，且circHIPK3-轴可能是ESCC患者的一个治疗靶点。
