特异性 LINC01146，一种有前途的预后指标，在体外和体内均抑制肝癌细胞的恶性表型。
Liver-specific LINC01146, a promising prognostic indicator, inhibits the malignant phenotype of hepatocellular carcinoma cells both in vitro and in vivo.
机构信息
Department of Epidemiology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, China.
Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.
出版信息
J Transl Med. 2022 Jan 31;20(1):57. doi: 10.1186/s12967-021-03225-2.
BACKGROUND
Long non-coding RNAs (lncRNAs) are involved in the development of hepatocellular carcinoma (HCC). We aimed to investigate the function of LINC01146 in HCC.
METHODS
The expression of LINC01146 in HCC tissues was explored via The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and was verified using quantitative real-time polymerase chain reaction (qRT-PCR) in our HCC cohort. Kaplan-Meier analysis was used to assess the relationship between LINC01146 and the prognosis of HCC patients. Cell Counting Kit 8, colony formation assays, Transwell assays, flow cytometric assays, and tumour formation models in nude mice were conducted to reveal the effects of LINC01146 on HCC cells both in vitro and in vivo. Bioinformatic methods were used to explore the possible potential pathways of LINC01146 in HCC.
RESULTS
LINC01146 was significantly decreased in HCC tissues compared with adjacent normal tissues and was found to be related to the clinical presentations of malignancy and the poor prognosis of HCC patients. Overexpression of LINC01146 inhibited the proliferation, migration, and invasion of HCC cells in vitro, while promoting their apoptosis. In contrast, downregulation of LINC01146 exerted the opposite effects on HCC cells in vitro. In addition, overexpression of LINC01146 significantly inhibited tumour growth, while downregulation of LINC01146 promoted tumour growth in vivo. Furthermore, the coexpressed genes of LINC01146 were mainly involved in the "metabolic pathway" and "complement and coagulation cascade pathway".
CONCLUSION
LINC01146 expression was found to be decreased in HCC tissues and associated with the prognosis of HCC patients. It may serve as a cancer suppressor and prognostic biomarker in HCC.
背景
长链非编码 RNA（lncRNA）参与肝癌（HCC）的发生发展。本研究旨在探讨 LINC01146 在 HCC 中的作用。
方法
通过癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）数据库探讨 HCC 组织中 LINC01146 的表达，并采用实时定量聚合酶链反应（qRT-PCR）在 HCC 队列中进行验证。Kaplan-Meier 分析评估 LINC01146 与 HCC 患者预后的关系。细胞计数试剂盒 8（CCK-8）、集落形成实验、Transwell 实验、流式细胞术以及裸鼠肿瘤形成模型实验用于体外和体内揭示 LINC01146 对 HCC 细胞的影响。生物信息学方法用于探讨 LINC01146 在 HCC 中潜在的可能通路。
结果
LINC01146 在 HCC 组织中显著下调，与邻近正常组织相比差异具有统计学意义，并且与 HCC 患者的临床恶性表现和不良预后相关。体外过表达 LINC01146 抑制 HCC 细胞的增殖、迁移和侵袭，促进其凋亡。相反，下调 LINC01146 对 HCC 细胞的体外表型发挥相反作用。此外，过表达 LINC01146 显著抑制肿瘤生长，而下调 LINC01146 则促进肿瘤生长。进一步分析 LINC01146 的共表达基因主要参与“代谢途径”和“补体和凝血级联途径”。
结论
LINC01146 在 HCC 组织中表达下调，与 HCC 患者的预后相关。它可能作为 HCC 的抑癌基因和预后生物标志物。
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