从蝙蝠粪便中分离得到的两株耐盐放线菌——高富汤姆替拉菌和封子介汤姆替拉菌的形态学和基因组特征。
Morphological and genomic characteristics of two novel halotolerant actinomycetes, Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. isolated from bat faeces.
机构信息
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China; Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China; Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, PR China.
出版信息
Syst Appl Microbiol. 2022 Apr;45(2):126294. doi: 10.1016/j.syapm.2022.126294. Epub 2022 Jan 19.
Four white-pigmented, Gram-staining-positive, strictly aerobic, non-spore-forming, irregular rod-shaped bacteria were isolated from the faeces of bats collected from Guangxi autonomous region (22°20'54″N, 106°49'20″E; July 28, 2011) and Chongqing city (30°02'15″N, 107°07'4″E; September 1, 2011) of South China. The strains shared 99.3-99.9% 16S rRNA gene sequence similarity by BLAST search among them, and belonged to genus Tomitella according to 16S rRNA gene and genomic sequence-based phylogenetic/phylogenomic analyses. Strains HY172 and HY188 contained meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose, glucose, galactose or ribose in their whole-cell hydrolysates. Besides sharing phosphatidylethanolamine, diphosphatidylglycerol and unidentified glycolipid(s) in their polar lipid profiles, additionally HY172 had one unidentified phosphoglycolipid and three unidentified phospholipids whereas HY188 had phosphatidyl inositol mannoside and four unidentified aminolipids. The main cellular fatty acids of strains HY172 and HY188 were C, C 10-methyl, Cω9c and summed feature 3. The genomic DNA G + C contents of both strains (HY172 and HY188) were 70.9 %. The genus Tomitella contains 2311 core genes, and resuscitation promoting factor (rpf) genes can be found in all members of Tomitella. The digital DNA-DNA hybridization and average nucleotide identity values of the four novel strains with other members of the genus Tomitella were within the ranges of 20.1-45.2% and 74.8-91.9%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point. Based on phylogenetic, chemotaxonomic and phenotypic analyses, these four strains could be classified as two novel species of the genus Tomitella, for which the names Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. are proposed. The type strains are HY172 (= CGMCC 1.18701 = JCM 34231) and HY188 (= CGMCC 1.16971 = JCM 33467), respectively.
从中国华南地区广西壮族自治区（22°20'54″N，106°49'20″E；2011 年 7 月 28 日）和重庆市（30°02'15″N，107°07'4″E；2011 年 9 月 1 日）采集的蝙蝠粪便中分离到 4 株白色、革兰氏阳性、严格需氧、非孢子形成、不规则杆状细菌。通过 BLAST 搜索，菌株之间的 16S rRNA 基因序列相似度为 99.3-99.9%，根据 16S rRNA 基因和基于基因组序列的系统发育/系统基因组分析，属于 Tomitella 属。菌株 HY172 和 HY188 含有中-二氨基庚二酸作为诊断二氨基酸，其全细胞水解物中含有阿拉伯糖、葡萄糖、半乳糖或核糖。除了在极性脂质图谱中共享磷脂酰乙醇胺、双磷脂酰甘油和未鉴定的糖脂外，HY172 还含有一种未鉴定的磷酸糖脂和三种未鉴定的磷脂，而 HY188 则含有磷脂酰肌醇甘露糖苷和四种未鉴定的胺脂。菌株 HY172 和 HY188 的主要细胞脂肪酸为 C、C10-甲基、Cω9c 和总和特征 3。两株菌（HY172 和 HY188）的基因组 DNA G+C 含量均为 70.9%。Tomitella 属包含 2311 个核心基因，所有 Tomitella 属成员都可以找到复苏促进因子（rpf）基因。与 Tomitella 属其他成员相比，这四个新菌株的数字 DNA-DNA 杂交和平均核苷酸同一性值分别在 20.1-45.2%和 74.8-91.9%之间，均低于各自推荐的 70.0%和 95-96%的截止值。基于系统发育、化学分类和表型分析，这四株菌可被分类为 Tomitella 属的两个新种，分别命名为 Tomitella gaofuii sp. nov. 和 Tomitella fengzijianii sp. nov.。模式菌株分别为 HY172（=CGMCC 1.18701=JCM 34231）和 HY188（=CGMCC 1.16971=JCM 33467）。
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