State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province, 030001, China.
J Microbiol. 2022 Oct;60(10):977-985. doi: 10.1007/s12275-022-2134-8. Epub 2022 Aug 19.
Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170 and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively. Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]). The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739 (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%). Strain HY170 contained MK-8(H), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively. The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170. Anteiso-C and anteiso-C were the major fatty acids (> 10%) of strains HY170 and HY001, with anteiso-CA predominant in strain HY170 but absent in strain HY001. Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene. Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp. nov. is proposed (type strain HY170 = CGMCC 1.18600 = JCM 34230).
两株严格需氧、革兰氏阳性、不产芽孢、规则棒状（约 0.7×1.9mm）的细菌（HY170 和 HY001）分别从广西壮族自治区崇左市（22°20'54″N，106°49'20″E，2011 年 7 月）和云南省楚雄彝族自治州（25°09'10″N，102°04'39″E，2013 年 10 月）的蝙蝠粪便中分离得到。最适生长温度为 25-28°C（范围为 4-32°C），在 BHI-5%绵羊血平板上，pH 值为 7.5（范围为 5.0-10.0），并在 0.5-1.0% NaCl（w/v）（范围为 0-15% NaCl [w/v]）的存在下。基于 16S rRNA 基因和 845 个核心基因序列构建的系统发育树和系统基因组树表明，这两株菌在短杆菌属内形成了一个独特的谱系，与橙色短杆菌 NCDO 739（16S rRNA 相似性均为 98.5%；dDDH，46.7-46.8%；ANI，91.9-92.1%）最为密切相关。菌株 HY170 含有 MK-8(H)、二磷酸甘油（DPG）和磷脂酰甘油（PG）、半乳糖和核糖分别作为主要的膜醌、主要极性脂和细胞壁磷壁酸中的主要糖。菌株 HY170 中的特征二氨基庚二酸（meso-DAP）是鉴定肽聚糖的二氨基庚二酸。菌株 HY170 和 HY001 的主要脂肪酸（>10%）为 anteiso-C 和 anteiso-C，菌株 HY170 以 anteiso-CA 为主，而菌株 HY001 中则不存在 anteiso-CA。基因组挖掘表明，存在编码非-α聚氨基酸（NAPAA）、ectoine、铁载体和萜烯的次生代谢物生物合成基因簇。基于系统发育、化学分类学和表型分析的结果，这两株菌可被归类为短杆菌属的一个新种，命名为短杆菌周鹏氏菌 sp. nov.（模式菌株 HY170=CGMCC 1.18600=JCM 34230）。
