Yue B Y, Hsieh P, Baum J L
Invest Ophthalmol Vis Sci. 1986 Jan;27(1):14-9.
Rabbit corneal tissues were treated sequentially with phosphate buffered saline (PBS) and 4 M guanidine hydrochloride (GuHCl), dialyzed, and lyophilized. The interaction of the individual PBS and the GuHCl extracts with cultured rabbit corneal stromal cells was assessed. The PBS extract stimulated stromal cell growth. These cells had a thinner spindle-shaped appearance, a greater tendency toward multilayer formation, and a approximately 40-60% higher final density than the controls. The cells subjected to the GuHCl extract exhibited no such changes. When the PBS extract was heated to 80 degrees C, the stimulatory activity was replaced by an inhibitory activity, indicating that the PBS extract contained both the stimulatory and the inhibitory factors. Using a high performance liquid chromatograph system, such factors could be separated. The effects of corneal extracts on connective tissue synthesis were examined after labeling confluent stromal cultures with either (35S)sulfate or (3H)proline for 20 hr. The PBS and the GuHCl extracts significantly promoted the incorporation of (35S)sulfate into glycosaminoglycans. Neither extract altered the types of glycosaminoglycans synthesized or the collagen synthesis of stromal keratocytes in culture.
兔角膜组织依次用磷酸盐缓冲盐水（PBS）和4M盐酸胍（GuHCl）处理，透析后冻干。评估了单独的PBS提取物和GuHCl提取物与培养的兔角膜基质细胞的相互作用。PBS提取物刺激基质细胞生长。这些细胞呈较细的纺锤形外观，形成多层的趋势更大，最终密度比对照高约40-60%。接受GuHCl提取物处理的细胞未表现出此类变化。当PBS提取物加热至80℃时，刺激活性被抑制活性取代，表明PBS提取物同时含有刺激因子和抑制因子。使用高效液相色谱系统可以分离这些因子。在用（35S）硫酸盐或（3H）脯氨酸标记汇合的基质培养物20小时后，检测角膜提取物对结缔组织合成的影响。PBS和GuHCl提取物均显著促进（35S）硫酸盐掺入糖胺聚糖。两种提取物均未改变培养的基质角膜细胞合成的糖胺聚糖类型或胶原蛋白合成。
