低心肺适能健康个体的致动脉脂质组学特征：HUNT3 研究。

Atherogenic lipidomics profile in healthy individuals with low cardiorespiratory fitness: The HUNT3 fitness study.

机构信息

Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway.

Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; Department of Cardiology, St. Olavs Hospital, Trondheim, Norway.

出版信息

Atherosclerosis. 2022 Feb;343:51-57. doi: 10.1016/j.atherosclerosis.2022.01.001. Epub 2022 Jan 20.

DOI:10.1016/j.atherosclerosis.2022.01.001

PMID:35101845

Abstract

BACKGROUND AND AIMS

Low cardiorespiratory fitness is a strong and independent risk factor for cardiovascular disease (CVD). Serum profiling of healthy individuals with large differences in cardiorespiratory fitness may therefore reveal early biomarkers of CVD development. Thus, we aimed to identify circulating lipoprotein subfractions differentially expressed between groups of individuals with large differences in cardiorespiratory fitness, measured as maximal oxygen uptake (VO).

METHODS

Healthy subjects (40-59 years) were selected from the third wave of the Trøndelag health study (HUNT3) based on having an age-dependent high VO (47.1 ± 7.7 mL kg·min, n = 103) or low VO (31.4 ± 4.9 mL kg·min, n = 108). The individuals were matched on gender, age, physical activity level and fasting status.

RESULTS

99 lipoprotein subfractions were quantified in serum samples using nuclear magnetic resonance (NMR) lipidomics. Standard clinical analyses showed similar levels of total cholesterol, low-density lipoprotein (LDL)-cholesterol and high-density lipoprotein (HDL)-cholesterol between the groups, and slightly higher levels of triglycerides in participants with low VO. Thirteen lipoprotein subfractions were increased in the low VO group compared to the high VO group (p < 0.005), including mainly large very low-density lipoprotein (VLDL) subfractions. In addition, triglyceride levels in small-sized HDL and LDL particles were increased in participants with low VO. Correlation analyses between VO and lipoproteins subfractions displayed a negative correlation between VO and the levels of cholesterol and phospholipids in the small HDL particles. The lipoprotein profile of individuals with low VO is similar to the profile of insulin resistant individuals.

CONCLUSIONS

Low VO was associated with enrichment of large VLDL particles, as well as an increased triglycerides content in the small and dense HDL and LDL particles. This unfavorable lipid profile is likely to be involved in the strong associations between VO and CVD.

摘要

背景与目的

心肺功能较差是心血管疾病（CVD）的一个强烈且独立的危险因素。因此，对心肺功能差异较大的健康个体进行血清分析可能会发现 CVD 发展的早期生物标志物。因此，我们旨在确定心肺功能差异较大的个体之间差异表达的循环脂蛋白亚组分，心肺功能通过最大摄氧量（VO）来衡量。

方法

根据年龄相关的高 VO（47.1±7.7mL·kg·min，n=103）或低 VO（31.4±4.9mL·kg·min，n=108），从特隆赫姆健康研究（HUNT3）的第三波中选择健康受试者（40-59 岁）。个体按性别、年龄、身体活动水平和禁食状态进行匹配。

结果

使用核磁共振（NMR）脂质组学对血清样本中的 99 种脂蛋白亚组分进行定量分析。标准临床分析显示，两组之间的总胆固醇、低密度脂蛋白（LDL）-胆固醇和高密度脂蛋白（HDL）-胆固醇水平相似，而低 VO 组的甘油三酯水平略高。与高 VO 组相比，低 VO 组有 13 种脂蛋白亚组分升高（p<0.005），主要包括大的极低密度脂蛋白（VLDL）亚组分。此外，低 VO 组的小尺寸 HDL 和 LDL 颗粒中的甘油三酯水平升高。VO 与脂蛋白亚组分之间的相关分析显示，VO 与小 HDL 颗粒中胆固醇和磷脂的水平呈负相关。低 VO 个体的脂蛋白谱与胰岛素抵抗个体的谱相似。