Dzau V J, Mudgett-Hunter M, Haber E
J Clin Endocrinol Metab. 1986 Feb;62(2):424-8. doi: 10.1210/jcem-62-2-424.
Monoclonal antibodies may be helpful in comparing the structural homology of renins of various animal species as well as other aspartyl proteinases. We obtained four hybridoma lines secreting monoclonal antibodies that inhibited 3 Goldblatt mU human renin enzymatic activity with apparent IC50 of 1.1 X 10(-6)-4 X 10(-8) M. Each antibody was specific for human renin and did not cross-react either with other proteins tested nor with renins of other species in an immunoradiometric assay. These antibodies did not inhibit the enzymatic activities of nonprimate renins or nonrenin aspartyl proteinases tested. These data indicate that certain structural determinants of human renin activity may be uniquely different from those of nonprimate renins or nonrenin aspartyl proteinases.
单克隆抗体可能有助于比较各种动物物种肾素以及其他天冬氨酸蛋白酶的结构同源性。我们获得了四个分泌单克隆抗体的杂交瘤细胞系，这些抗体抑制3 Goldblatt mU人肾素酶活性，表观IC50为1.1×10(-6)-4×10(-8)M。每种抗体对人肾素具有特异性，在免疫放射分析中既不与测试的其他蛋白质发生交叉反应，也不与其他物种的肾素发生交叉反应。这些抗体不抑制所测试的非灵长类动物肾素或非肾素天冬氨酸蛋白酶的酶活性。这些数据表明，人肾素活性的某些结构决定因素可能与非灵长类动物肾素或非肾素天冬氨酸蛋白酶的结构决定因素有独特的不同。
