Department of Medical Oncology and Cancer Center, Shiga University of Medical Science, Otsu, Shiga 520‑2192, Japan.
Department of Oral and Maxillofacial Surgery, Kumamoto University, Kumamoto 860‑8555, Japan.
Int J Oncol. 2022 Mar;60(3). doi: 10.3892/ijo.2022.5317. Epub 2022 Feb 1.
Oral cancer is a leading cause of cancer‑related death worldwide. Current treatment for oral cancer includes surgery, radiotherapy, and chemotherapy; however, their effectiveness is still limited. To identify a new prognostic biomarker and therapeutic target for oral cancer, the Opa interacting protein 5 (OIP5), which plays an essential role in the proper segregation of chromosomes, was examined. Immunohistochemical staining using tissue microarrays indicated that OIP5 was expressed in 120 of 164 (73.2%) oral cancers but was minimally expressed in normal oral tissues. OIP5 expression was significantly associated with poor prognosis in patients with oral cancer. Overexpression of OIP5 enhanced the growth of oral cancer cells, whereas OIP5 knockdown using small interfering RNAs (siRNAs) significantly inhibited cell growth through cell cycle arrest at the G2/M phase. Suppression of OIP5 expression also induced senescence of oral cancer cells. Overall, the findings of the present study suggest that OIP5 may be a candidate prognostic biomarker and therapeutic target in oral cancer.
口腔癌是全球癌症相关死亡的主要原因之一。目前口腔癌的治疗包括手术、放疗和化疗；然而，它们的疗效仍然有限。为了确定口腔癌的新预后生物标志物和治疗靶点，本研究检测了在染色体正确分离中起关键作用的 Opa 相互作用蛋白 5（OIP5）。使用组织微阵列的免疫组织化学染色表明，OIP5 在 164 例口腔癌中的 120 例（73.2%）中表达，但在正常口腔组织中表达极少。OIP5 的表达与口腔癌患者的不良预后显著相关。OIP5 的过表达增强了口腔癌细胞的生长，而使用小干扰 RNA（siRNA）进行 OIP5 敲低则通过细胞周期阻滞在 G2/M 期显著抑制细胞生长。抑制 OIP5 的表达也诱导了口腔癌细胞衰老。总的来说，本研究的结果表明，OIP5 可能是口腔癌的候选预后生物标志物和治疗靶点。
