Faculty of Chemistry, Alexandru Ioan Cuza University of Iasi, 11 Carol I, Iasi, 700506, Romania.
TRANSCEND Research Center, Regional Institute of Oncology, General Henri Mathias, No. 2-4, Iasi, 700483, Romania.
Chempluschem. 2022 Feb;87(2):e202100462. doi: 10.1002/cplu.202100462.
Bioinspired peptides are attractive biomolecules which can improve our understanding of self-assembly processes for rational design of new peptide-based materials. Herein, a new amidated peptide FRSAPFIE (FRS), based on a sequence present in human collagen, was synthesized, characterized by mass spectrometry and subjected to self-assembling investigations. The optimal conditions for self-assembly were disclosed by dynamic light scattering at 32 °C and a peptide concentration of 0.51 %. In addition, AFM studies revealed ellipsoidal FRS shapes with an area between 0.8 and 3.1 μm . The ability of self-assembly was also proved using FAD dye as extrinsic fluorescence reporter. According to the theoretical analysis, the FRS peptide tends to form a bundle-type association, with a type of fibrillary tangles particle. Altogether, our findings address new challenges regarding the FRS peptide which can be used in further self-assembly studies to design biocompatible drug-delivery platforms.
仿生肽是一种有吸引力的生物分子，它可以增进我们对自组装过程的理解，从而实现基于肽的新型材料的合理设计。在此，我们合成了一种新的酰胺化肽 FRSAPFIE（FRS），该肽基于人胶原蛋白中的一个序列，通过质谱进行了表征，并进行了自组装研究。通过在 32°C 和肽浓度为 0.51%的条件下进行动态光散射，揭示了自组装的最佳条件。此外，原子力显微镜研究显示 FRS 形状为椭圆形，面积在 0.8 到 3.1μm 之间。使用 FAD 染料作为外源性荧光报告也证明了自组装的能力。根据理论分析，FRS 肽倾向于形成束状缔合，形成一种纤维缠结颗粒。总之，我们的研究结果为 FRS 肽提出了新的挑战，可用于进一步的自组装研究，以设计生物相容性的药物递送平台。
