Lieske Camilla L, Herndon David R, Highland Margaret A, Beckmen Kimberlee B
Alaska Department of Fish and Game, Division of Wildlife Conservation, 1300 College Road, Fairbanks, Alaska 99701-1551, USA.
US Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, ADBF 3003, Pullman, Washington 99164-0001, USA.
J Wildl Dis. 2022 Apr 1;58(2):257-268. doi: 10.7589/JWD-D-21-00118.
As part of a respiratory pathogen survey of Alaska wildlife, we conducted a concordance study to assess Mycoplasma ovipneumoniae detection among three different PCR assays using a total of 346 nasal swabs sampled from four species (Dall's sheep, Ovis dalli dalli; mountain goats, Oreamnos americanus; caribou, Rangifer tarandus granti; and moose, Alces alces gigas), and two taxonomic subfamilies (Bovidae subfamily Caprinae and Cervidae subfamily Capreolinae). A federal research laboratory performed two PCR assays (LM40 and intergenic spacer region [IGS]), and a state diagnostic laboratory performed the third (universal Mycoplasma [UM]). Overall concordance was good, ranging from 93% to 99%, which was probably a result of low detection rate of M. ovipneumoniae. Due to differences in positive agreement, the quality of concordance between LM40 and both IGS and UM was considered fair. However, the quality of concordance between IGS and UM was excellent. All three PCR methods detected M. ovipneumoniae in a non-Caprinae species (caribou), and the LM40-PCR assay also detected M. ovipneumoniae in additional Caprinae species. The LM40-PCR assay detected M. ovipneumoniae in a larger number of samples than did the other two assays (IGS, UM). Because of potential differences in detection rates, it is critical to consider test parameters when evaluating a host population for the presence of M. ovipneumoniae.
作为阿拉斯加野生动物呼吸道病原体调查的一部分，我们进行了一项一致性研究，以评估三种不同的聚合酶链反应（PCR）检测方法对绵羊肺炎支原体的检测情况。我们总共采集了来自四个物种（多尔大角羊，Ovis dalli dalli；北美野山羊，Oreamnos americanus；北美驯鹿，Rangifer tarandus granti；驼鹿，Alces alces gigas）以及两个分类亚科（牛科羊亚科和鹿科麂亚科）的346份鼻拭子样本。一个联邦研究实验室进行了两种PCR检测方法（LM40和基因间隔区[IGS]），一个州诊断实验室进行了第三种检测方法（通用支原体[UM]）。总体一致性良好，范围在93%至99%之间，这可能是绵羊肺炎支原体检出率较低的结果。由于阳性一致性存在差异，LM40与IGS和UM之间的一致性质量被认为一般。然而，IGS和UM之间的一致性质量非常好。所有三种PCR方法都在一个非羊亚科物种（北美驯鹿）中检测到了绵羊肺炎支原体，并且LM40-PCR检测方法还在其他羊亚科物种中检测到了绵羊肺炎支原体。LM40-PCR检测方法检测到绵羊肺炎支原体的样本数量比其他两种检测方法（IGS、UM）更多。由于检测率可能存在差异，在评估宿主群体中是否存在绵羊肺炎支原体时，考虑检测参数至关重要。
