Mandel G, Clark W
J Immunol. 1978 May;120(5):1637-43.
The effect of fatty acid substitution of membrane phosphatides on a number of surface-mediated phenomena in EL-4 cells was examined. Tumor cells were grown in the presence of fatty acids that could be expected, on the basis of their physical properties, either to stiffen or fluidize the plasma membrane. Substitution of EL-4 cell membrane phosphatides with as much as 74% nonadecanoic acid (19:0) had no effect on either conjugation with effector cells or subsequent cytolysis by the effector cells. Substitution with linolenic acid (18:3) or elaidic acid (18:1trans) likewise affected neither conjugation nor cytolysis. Substitution with these fatty acids also had no effect on the susceptibility of EL-4 cells to cytolysis by antibody plus complement. On the other hand, the rate of patching of H-2 surface antigens was very sensitive to substitution by both 19:0 and 18:3. Although not conclusive, these results suggest that alterations of the fluid state of the membrane that affect lateral movements of surface proteins may not affect cytolytic processes.
研究了膜磷脂的脂肪酸取代对EL-4细胞中一些表面介导现象的影响。肿瘤细胞在脂肪酸存在的情况下生长，基于其物理性质，这些脂肪酸预期会使质膜变硬或使其流化。用高达74%的十九烷酸（19:0）取代EL-4细胞膜磷脂，对与效应细胞的结合或效应细胞随后的细胞溶解均无影响。用亚麻酸（18:3）或反油酸（18:1反式）取代同样既不影响结合也不影响细胞溶解。用这些脂肪酸取代对EL-4细胞对抗体加补体细胞溶解的敏感性也没有影响。另一方面，H-2表面抗原的成斑速率对19:0和18:3的取代非常敏感。尽管尚无定论，但这些结果表明，影响表面蛋白侧向运动的膜流体状态改变可能不会影响细胞溶解过程。
