Dreno B, Milpied N, Harousseau J L, Stalder J F, Bureau B, Milpied B, Barriere H
Br J Dermatol. 1986 Jan;114(1):7-15. doi: 10.1111/j.1365-2133.1986.tb02774.x.
A comparative study of the healthy skin of patients who had undergone bone marrow grafting and not developed graft-versus-host disease (GVHD) and of patients with cutaneous lesions of acute GVHD has been carried out. The aim of this study was to assess the diagnostic value of cutaneous immunopathology in the diagnosis of acute GVHD. A double-labelling immunofluorescence technique was used with a panel of monoclonal antibodies. The results showed a lack of specificity for GVHD in the distribution of Langerhans cells, but confirmed the diagnostic value of HLA-DR staining of epidermal keratinocytes. Cellular polymorphism of the T cell infiltrate in the dermis was observed (T helpers 40% and T suppressors 20%). The expression of the 55-57 Kd keratin polypeptide and of bullous pemphigoid antigen showed modification during acute GVHD while that of pemphigus antigen remained unchanged.
对接受骨髓移植且未发生移植物抗宿主病（GVHD）的患者的健康皮肤，以及患有急性GVHD皮肤病变的患者进行了一项比较研究。本研究的目的是评估皮肤免疫病理学在急性GVHD诊断中的诊断价值。使用一组单克隆抗体的双标记免疫荧光技术。结果显示，朗格汉斯细胞分布对GVHD缺乏特异性，但证实了表皮角质形成细胞HLA-DR染色的诊断价值。观察到真皮中T细胞浸润的细胞多态性（辅助性T细胞占40%，抑制性T细胞占20%）。在急性GVHD期间，55 - 57 Kd角蛋白多肽和大疱性类天疱疮抗原的表达发生改变，而天疱疮抗原的表达保持不变。
