McKinney R M, Thacker L, Wong M C, Hebert G A
J Immunol Methods. 1978;21(1-2):1-10. doi: 10.1016/0022-1759(78)90218-1.
Bacterial cell columns for immunoadsorption were prepared with Streptococcus cells and triethylaminoethyl cellulose (Cellex-T) matrix material as a model system. Good column flow properties and satisfactory retention of the cells were obtained with ratios as high as 2 ml of packed cells/3 g dry weight of cellulose. Anion-exchange fractionation of whole serum by the Cellex-T was prevented by using 0.25 M NaCl in the developing buffer. Antibodies were adsorbed directly from whole serum and recovered in high yield by desorption at pH 2.3. Pre-exposing bacterial cells to formalin and washing them with acetone was necessary to ensure that they remained on the columns. One strain of Streptococcus salivarius (SS 908) was satisfactorily retained on a column only after cells were labeled with fluorescein isothiocyanate and washed with acetone. The means by which Cellex-T retains bacterial cells appears to be a combination of electronic attraction and physical entrapment.
以链球菌细胞和三乙氨基乙基纤维素（Cellex - T）基质材料作为模型系统制备用于免疫吸附的细菌细胞柱。当比例高达2 ml填充细胞/3 g纤维素干重时，可获得良好的柱流动特性和令人满意的细胞保留率。通过在展开缓冲液中使用0.25 M NaCl可防止Cellex - T对全血清进行阴离子交换分级分离。抗体直接从全血清中吸附，并通过在pH 2.3下解吸以高产量回收。预先将细菌细胞用福尔马林处理并用丙酮洗涤，对于确保它们留在柱上是必要的。仅在细胞用异硫氰酸荧光素标记并用丙酮洗涤后，一株唾液链球菌（SS 908）才能令人满意地保留在柱上。Cellex - T保留细菌细胞的方式似乎是电子吸引和物理截留的结合。
