Highton J, Hessian P A, Small B, Palmer D G
Br J Rheumatol. 1986 Feb;25(1):20-5. doi: 10.1093/rheumatology/25.1.20.
Two enzyme immunoassays for rheumatoid factor (RF) were compared with the traditional latex agglutination test. Preference is expressed for an ELISA specific for IgM-RF because it yields specific, quantitative results. Variability of this assay was least in sera containing moderate levels of RF and it was less precise at low concentrations. Survey of a random selection from the local population showed a similar prevalence of IgM-RF positivity as revealed by previous surveys using agglutination techniques. We conclude that measurement by ELISA yields no great increase in the discriminative ability of RF testing.
将两种类风湿因子（RF）酶免疫测定法与传统乳胶凝集试验进行了比较。更倾向于使用针对IgM-RF的酶联免疫吸附测定（ELISA），因为它能产生特异的定量结果。该测定法在含有中等水平RF的血清中变异性最小，在低浓度时精度较低。对当地人群随机抽样调查显示，IgM-RF阳性率与之前使用凝集技术的调查结果相似。我们得出结论，ELISA测定法在RF检测的鉴别能力方面没有显著提高。
