Vejtorp M, Høier-Madsen M, Halberg P
Scand J Rheumatol. 1979;8(2):65-70. doi: 10.3109/03009747909105338.
A semi-automated enzyme-linked immunosorbent assay (ELISA) for determination of IgM rheumatoid factor was established. Human gammaglobulin (Cohn fraction II) was used as an antigen on a solid phase of polystyrene microtitre trays. The results were read on a spectrophotometer. ELISA and the sheep cell agglutination test were compared in a study of sera from 400 blood donors, 53 patients with active rheumatoid arthritis and of 200 sera received for routine determination of rheumatoid factor. The results of the two tests correlated well. The ELISA procedure was precise, objective, inexpensive and well suited for quantitative routine determination of IgM rheumatoid factor.
建立了一种用于测定 IgM 类风湿因子的半自动酶联免疫吸附测定法（ELISA）。用人丙种球蛋白（科恩 II 组分）作为聚苯乙烯微量滴定板固相上的抗原。结果在分光光度计上读取。在对 400 名献血者的血清、53 名活动性类风湿性关节炎患者的血清以及 200 份用于类风湿因子常规测定的血清进行的研究中，对 ELISA 和绵羊细胞凝集试验进行了比较。两种试验的结果相关性良好。ELISA 方法精确、客观、成本低廉，非常适合 IgM 类风湿因子的定量常规测定。
