Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Anal Chem. 2022 Mar 15;94(10):4294-4302. doi: 10.1021/acs.analchem.1c04957. Epub 2022 Feb 2.
The detection of rosiglitazone (RSG) in food is of great importance since the excessive intake of RSG could cause adverse effects on the human body. Although liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry are the preliminary methods for the detection of hazardous materials in food, they are not suitable for point-of-care or on-site detection. Herein, a time-based readout (TBR) device with an application software (APP) controlled by a smart phone was developed for the sensitive and selective immunoassay of RSG. The homemade TBR device was based on a two-electrode system, where the immune molecule-modified glassy carbon electrode was used as the bioanode, and Prussian blue-modified FTO was used as the cathode. By using Au-modified octahedral CuO with high catalytic activity as mimetic peroxidase, an insulating layer was generated on the cathode by catalyzing 4-chloro-1-naphthol (4-CN) into benzo-4-chlorohexadienone (B4Q). The time to reach a fixed potential varied indirectly with the concentrations of RSG and was recognized by the APP, while the electrochromic property on the cathode was also correspondingly changed. Under optimum conditions, both the square root of the time and the chroma value of the electrochromism exhibited linear responses for the detection of RSG ranging from 5 × 10 to 5 × 10 g/L, while the limits of detection were 8.2 × 10 and 1.3 × 10 g/L, respectively. With easy operation and portability, this TBR device showed a promising application for point-of-care monitoring of hazardous materials in food or the environment.
罗格列酮（RSG）的检测在食品中非常重要，因为过量摄入 RSG 可能会对人体造成不良影响。虽然液相色谱-质谱法和气相色谱-质谱法是检测食品中有害物质的初步方法，但它们不适合现场或现场检测。本文开发了一种基于时间读取（TBR）的设备，该设备带有智能手机控制的应用软件（APP），用于 RSG 的灵敏和选择性免疫分析。自制的 TBR 设备基于双电极系统，其中免疫分子修饰的玻碳电极用作生物阳极，普鲁士蓝修饰的 FTO 用作阴极。通过使用具有高催化活性的 Au 修饰的八面体 CuO 作为模拟过氧化物酶，在阴极上通过催化 4-氯-1-萘酚（4-CN）生成苯并-4-氯己二烯酮（B4Q）生成绝缘层。达到固定电势的时间间接随 RSG 的浓度变化而变化，并由 APP 识别，同时阴极的电致变色性质也相应变化。在最佳条件下，RSG 的检测范围为 5×10 到 5×10 g/L 时，时间平方根和电致变色的色值均表现出线性响应，检出限分别为 8.2×10 和 1.3×10 g/L。该 TBR 设备操作简单，便携性好，有望用于现场监测食品或环境中有害物质。
