Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
Mikrochim Acta. 2022 Aug 3;189(8):312. doi: 10.1007/s00604-022-05415-1.
Due to the highly similar genetic background, it is difficult to distinguish Bacillus cereus (B. cereus) with other members of B. cereus group. Herein, an antibody-based colorimetric immunoassay using Cu-doped CeO nanospheres as peroxidase mimics was developed for the detection of B. cereus in food. First, monoclonal antibodies (mAbs) and polyclonal antibody (pAb) with good specificity to B. cereus were prepared and characterized. Second, the regular-shaped hollow Cu/CeO nanospheres with highly catalytic activity and biocompatibility were synthesized as mimic nanozymes to capture secondary antibody. Finally, a sandwich colorimetric immunoassay for the specific and sensitive detection of B. cereus was developed, showing linear detection range from 3.2 × 10 to 1 × 10 CFU/mL and a limit detection of 1.7 × 10 CFU/mL. The developed immunoassay holds great potential as an effective tool for detecting B. cereus in food poisoning.
由于具有高度相似的遗传背景，因此很难将蜡样芽胞杆菌（B. cereus）与芽孢杆菌属中的其他成员区分开来。在此，开发了一种基于抗体的比色免疫测定法，该方法使用掺铜的 CeO 纳米球作为过氧化物酶模拟物，用于检测食品中的蜡样芽胞杆菌。首先，制备并表征了对 B. cereus 具有良好特异性的单克隆抗体（mAb）和多克隆抗体（pAb）。其次，合成了具有高催化活性和生物相容性的规则形状的空心 Cu/CeO 纳米球作为模拟纳米酶来捕获二级抗体。最后，开发了一种用于特异性和灵敏检测 B. cereus 的夹心比色免疫测定法，其线性检测范围为 3.2×10 至 1×10 CFU/mL，检测限为 1.7×10 CFU/mL。该免疫测定法具有很大的潜力，可作为食物中毒中检测 B. cereus 的有效工具。
