Department of Biology, Johns Hopkins University, 3400 N. Charles Street, 200 Mudd Hall, Baltimore, MD 21218, USA.
Department of Biology, Johns Hopkins University, 3400 N. Charles Street, 200 Mudd Hall, Baltimore, MD 21218, USA; Section on Light and Circadian Rhythms (SLCR), National Institute of Mental Health, NIH, Bethesda, MD 20892, USA.
Cell Rep. 2022 Feb 1;38(5):110328. doi: 10.1016/j.celrep.2022.110328.
Satellite glia are the major glial type found in sympathetic and sensory ganglia in the peripheral nervous system, and specifically, contact neuronal cell bodies. Sympathetic and sensory neurons differ in morphological, molecular, and electrophysiological properties. However, the molecular diversity of the associated satellite glial cells remains unclear. Here, using single-cell RNA sequencing analysis, we identify five different populations of satellite glia from sympathetic and sensory ganglia. We define three shared populations of satellite glia enriched in immune-response genes, immediate-early genes, and ion channels/ECM-interactors, respectively. Sensory- and sympathetic-specific satellite glia are differentially enriched for modulators of lipid synthesis and metabolism. Sensory glia are also specifically enriched for genes involved in glutamate turnover. Furthermore, satellite glia and Schwann cells can be distinguished by unique transcriptional signatures. This study reveals the remarkable heterogeneity of satellite glia in the peripheral nervous system.
卫星胶质细胞是外周神经系统交感神经和感觉神经节中主要的神经胶质细胞类型，特别是与神经元细胞体接触。交感神经和感觉神经元在形态、分子和电生理特性上有所不同。然而，相关卫星胶质细胞的分子多样性尚不清楚。在这里，我们使用单细胞 RNA 测序分析，从交感神经和感觉神经节中鉴定出五种不同的卫星胶质细胞群体。我们分别定义了三种共享的卫星胶质细胞群体，它们分别富含免疫反应基因、即刻早期基因和离子通道/细胞外基质相互作用因子。感觉和交感神经特异性卫星胶质细胞分别富含脂质合成和代谢调节剂。感觉胶质细胞还特异性地富含涉及谷氨酸周转的基因。此外，卫星胶质细胞和雪旺细胞可以通过独特的转录特征来区分。本研究揭示了外周神经系统卫星胶质细胞的显著异质性。
