Shek P N, Lopez N G, Heath T D
Immunology. 1986 Jan;57(1):153-7.
Large unilamellar reverse-phase evaporation vesicles (REV) were used as a heterobifunctional carrier for a pharmacologically active agent and a protein antigen. Methotrexate (MTX) was encapsulated in the hydrophilic compartment of liposomal REV with or without surface-conjugated bovine serum albumin (BSA) antigen. The administration of MTX encapsulated within BSA-coated vesicles (MTX.REV-BSA) could either enhance or depress the anti-BSA plaque-forming cell (PFC) response in mice. On the other hand, the administration of MTX entrapped in plain vesicles (MTX.REV) produced essentially a suppressive effect on the PFC response stimulated by the simultaneous injection of a separate antigen, e.g. vesicle-conjugated BSA (REV-BSA). This suppression of the antibody response occurred, whether MTX.REV was injected 1 day before, during, or 1 day after the immunization with an antigen. These results indicate that liposome-encapsulation of a very limited drug dose can modify the immunobiological effect of the drug which, when presented in its free form at the same dose, exerts no appreciable action on the engendered immune response. Thus, the modulation of the immune response by a liposome-encapsulated drug appears to be influenced by the drug dose can modify the immunobiological effect of the drug which, when presented in its free form.
大单层反相蒸发囊泡（REV）被用作药理活性剂和蛋白质抗原的异双功能载体。甲氨蝶呤（MTX）被包裹在脂质体REV的亲水隔室中，脂质体表面有或没有偶联牛血清白蛋白（BSA）抗原。给予包裹在BSA包被囊泡中的MTX（MTX.REV-BSA）可增强或抑制小鼠抗BSA空斑形成细胞（PFC）反应。另一方面，给予包裹在普通囊泡中的MTX（MTX.REV）对同时注射单独抗原（如囊泡偶联的BSA，REV-BSA）刺激的PFC反应产生基本的抑制作用。无论MTX.REV是在抗原免疫前1天、免疫期间还是免疫后1天注射，抗体反应的这种抑制都会发生。这些结果表明，极少量药物剂量的脂质体包裹可以改变药物的免疫生物学效应，而相同剂量的游离形式药物对产生的免疫反应没有明显作用。因此，脂质体包裹药物对免疫反应的调节似乎受药物剂量的影响，药物剂量可以改变药物的免疫生物学效应，而药物以游离形式存在时则不然。
