Shek P N, Lukovich S
Immunol Lett. 1982;5(6):305-9. doi: 10.1016/0165-2478(82)90118-3.
The effect of the in vivo depletion of macrophage function on the plaque-forming cell (PFC) response to liposome-associated bovine serum albumin (LSM-BSA) in mice was investigated. Suppression of macrophage activities was accomplished by the intraperitoneal administration of carrageenan (CGN), a sulphated polygalactose which selectively inhibits macrophage function without interfering with the reactivity of T- and B-lymphocytes. Thus, control animals injected with CGN were found to give a significantly suppressed PFC response to sheep red blood cells, a macrophage-dependent antigen, but not to E. coli lipopolysaccharide, a macrophage-independent antigen. The pretreatment of experimental animals with CGN virtually abolished their antibody response to LSM-BSA in terms of the circulating anti-BSA antibody titers and the BSA-specific PFC response. These results provide evidence to substantiate an obligatory role of macrophages in inducing the humoral response to liposome-associated protein antigens.
研究了体内巨噬细胞功能耗竭对小鼠针对脂质体结合牛血清白蛋白（LSM-BSA）的空斑形成细胞（PFC）反应的影响。通过腹腔注射角叉菜胶（CGN）来抑制巨噬细胞活性，角叉菜胶是一种硫酸化的多聚半乳糖，可选择性抑制巨噬细胞功能而不干扰T淋巴细胞和B淋巴细胞的反应性。因此，发现注射CGN的对照动物对绵羊红细胞（一种巨噬细胞依赖性抗原）的PFC反应明显受到抑制，但对大肠杆菌脂多糖（一种巨噬细胞非依赖性抗原）的反应未受抑制。就循环抗BSA抗体滴度和BSA特异性PFC反应而言，用CGN对实验动物进行预处理实际上消除了它们对LSM-BSA的抗体反应。这些结果提供了证据，证实巨噬细胞在诱导针对脂质体结合蛋白抗原的体液反应中起必不可少的作用。
