Shek P N, Sabiston B H
Immunology. 1982 Dec;47(4):627-32.
The immunogenicity of different preparations of liposome-associated bovine serum albumin (LSM-BSA) was compared in order to examine the significance of the site of association between a protein antigen and the liposomal carrier. Animals immunized with liposomes containing both entrapped and surface-adsorbed BSA. (LSM-BSA) e + a, were found to give a BSA-specific plaque-forming cell (PFC) response comparable with that elicited by animals immunized with trypsinized (LSM-BSA) e + a which contained mainly entrapped BSA. In contrast, the PFC response elicited by animals immunized with liposomes containing BSA adsorbed on the membrane surface, (LSM-BSA)a, was significantly less than that of the above two groups. Finally, animals injected with trypsinized (LSM-BSA)a only elicited a marginally detectable PFC response. Results of this study demonstrate that liposomes can enhance the antibody response of a protein antigen regardless of whether the antigen is entrapped within the vesicle or absorbed on the outer surface. Entrapped antigen, however, proved to be more immunogenic than that which was surface-adsorbed.
为了研究蛋白质抗原与脂质体载体之间结合位点的重要性，对不同制剂的脂质体相关牛血清白蛋白（LSM-BSA）的免疫原性进行了比较。用同时含有包封型和表面吸附型牛血清白蛋白的脂质体（LSM-BSA）e + a免疫的动物，其牛血清白蛋白特异性空斑形成细胞（PFC）反应与用主要含有包封型牛血清白蛋白的胰蛋白酶处理的（LSM-BSA）e + a免疫的动物所引发的反应相当。相比之下，用含有吸附在膜表面的牛血清白蛋白的脂质体（LSM-BSA）a免疫的动物所引发的PFC反应明显低于上述两组。最后，仅注射胰蛋白酶处理的（LSM-BSA）a的动物仅引发了勉强可检测到的PFC反应。这项研究的结果表明，脂质体可以增强蛋白质抗原的抗体反应，无论抗原是包封在囊泡内还是吸附在外表面。然而，事实证明，包封型抗原比表面吸附型抗原具有更强的免疫原性。
