• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

SARS-CoV-2 和 MERS-CoV 感染的原代人肾上皮细胞的蛋白质组学全景。

Proteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells.

机构信息

Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany.

Institute of Virology, Philipps University Marburg, Marburg, Germany.

出版信息

Life Sci Alliance. 2022 Feb 2;5(5). doi: 10.26508/lsa.202201371. Print 2022 May.

DOI:10.26508/lsa.202201371
PMID:35110370
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8814637/
Abstract

Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type-specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection-induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type-specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication.

摘要

急性肾损伤与 COVID-19 患者的死亡率相关。然而，SARS-CoV-2 感染肾细胞的宿主细胞变化尚不清楚，这阻碍了对可能导致肾病理的分子机制的理解。在这里，我们对来自人类供体的原代肾近端和远端肾小管上皮细胞进行了定量翻译组学和全细胞蛋白质组学分析，以传播病毒并随时间推移观察细胞类型特异性变化。我们的研究结果揭示了两种病毒感染后共同修饰的途径，以及 SARS-CoV-2 特异性宿主细胞调节，驱动固有免疫激活和细胞蛋白质质量控制的关键变化。值得注意的是，MERS-CoV 感染诱导的线粒体生物学的特定变化在 SARS-CoV-2 感染中没有观察到。此外，我们还发现与肾衰竭相关的途径发生了广泛的调节，这些调节以病毒和细胞类型特异性的方式发生。总之，我们提供了 SARS-CoV-2 或 MERS-CoV 感染对原代肾上皮细胞的影响概述，揭示了可能对病毒复制至关重要的关键途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/0bcf9c0a8c6f/LSA-2022-01371_FigS9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/79bb580c5767/LSA-2022-01371_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/c9e05493886a/LSA-2022-01371_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/4dc00f16b470/LSA-2022-01371_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/64e6f6c548a4/LSA-2022-01371_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/a7b4e502134b/LSA-2022-01371_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/394d11236350/LSA-2022-01371_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/6c481a87fa26/LSA-2022-01371_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/2c26b72f9b2a/LSA-2022-01371_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/d2bde3ce17f9/LSA-2022-01371_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/aee40f9cde0a/LSA-2022-01371_FigS6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/6cda92826c57/LSA-2022-01371_FigS7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/407e7f2aace1/LSA-2022-01371_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/900769b2dec2/LSA-2022-01371_FigS8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/96c92aa77f3c/LSA-2022-01371_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/0bcf9c0a8c6f/LSA-2022-01371_FigS9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/79bb580c5767/LSA-2022-01371_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/c9e05493886a/LSA-2022-01371_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/4dc00f16b470/LSA-2022-01371_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/64e6f6c548a4/LSA-2022-01371_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/a7b4e502134b/LSA-2022-01371_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/394d11236350/LSA-2022-01371_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/6c481a87fa26/LSA-2022-01371_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/2c26b72f9b2a/LSA-2022-01371_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/d2bde3ce17f9/LSA-2022-01371_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/aee40f9cde0a/LSA-2022-01371_FigS6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/6cda92826c57/LSA-2022-01371_FigS7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/407e7f2aace1/LSA-2022-01371_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/900769b2dec2/LSA-2022-01371_FigS8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/96c92aa77f3c/LSA-2022-01371_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61bc/8814637/0bcf9c0a8c6f/LSA-2022-01371_FigS9.jpg

相似文献

1
Proteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells.SARS-CoV-2 和 MERS-CoV 感染的原代人肾上皮细胞的蛋白质组学全景。
Life Sci Alliance. 2022 Feb 2;5(5). doi: 10.26508/lsa.202201371. Print 2022 May.
2
In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.体外肾上皮细胞感染揭示了病毒对肾脏的趋向性，这可能是中东呼吸综合征冠状病毒感染导致急性肾衰竭的机制之一。
Virol J. 2013 Dec 23;10:359. doi: 10.1186/1743-422X-10-359.
3
H2V: a database of human genes and proteins that respond to SARS-CoV-2, SARS-CoV, and MERS-CoV infection.H2V：一个人类基因和蛋白质数据库，可响应 SARS-CoV-2、SARS-CoV 和 MERS-CoV 感染。
BMC Bioinformatics. 2021 Jan 7;22(1):18. doi: 10.1186/s12859-020-03935-2.
4
The lethal internal face of the coronaviruses: Kidney tropism of the SARS, MERS, and COVID19 viruses.冠状病毒致命的内在一面：SARS、MERS 和 COVID19 病毒对肾脏的趋向性。
IUBMB Life. 2021 Aug;73(8):1005-1015. doi: 10.1002/iub.2516. Epub 2021 Jun 30.
5
The transcriptomic profiling of SARS-CoV-2 compared to SARS, MERS, EBOV, and H1N1.与 SARS、MERS、EBOV 和 H1N1 相比，SARS-CoV-2 的转录组特征分析。
PLoS One. 2020 Dec 10;15(12):e0243270. doi: 10.1371/journal.pone.0243270. eCollection 2020.
6
Proteomics of SARS-CoV-2-infected host cells reveals therapy targets.SARS-CoV-2 感染宿主细胞的蛋白质组学研究揭示了治疗靶点。
Nature. 2020 Jul;583(7816):469-472. doi: 10.1038/s41586-020-2332-7. Epub 2020 May 14.
7
Gene expression pattern differences in primary human pulmonary epithelial cells infected with MERS-CoV or SARS-CoV-2.感染中东呼吸综合征冠状病毒（MERS-CoV）或严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的原代人肺上皮细胞中的基因表达模式差异
Arch Virol. 2020 Oct;165(10):2205-2211. doi: 10.1007/s00705-020-04730-3. Epub 2020 Jul 10.
8
The N-Terminal Region of Middle East Respiratory Syndrome Coronavirus Accessory Protein 8b Is Essential for Enhanced Virulence of an Attenuated Murine Coronavirus.中东呼吸综合征冠状病毒辅助蛋白 8b 的 N 端区域对于减毒鼠冠状病毒增强毒力是必需的。
J Virol. 2022 Feb 9;96(3):e0184221. doi: 10.1128/JVI.01842-21. Epub 2021 Nov 24.
9
HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV.HTCC 作为 SARS-CoV-2 和 MERS-CoV 的聚合抑制剂。
J Virol. 2021 Jan 28;95(4). doi: 10.1128/JVI.01622-20.
10
Middle East respiratory syndrome coronavirus infection: virus-host cell interactions and implications on pathogenesis.中东呼吸综合征冠状病毒感染：病毒与宿主细胞的相互作用及其对发病机制的影响
Virol J. 2015 Dec 22;12:218. doi: 10.1186/s12985-015-0446-6.

引用本文的文献

1
Mitochondria in COVID-19: from cellular and molecular perspective.新型冠状病毒肺炎中的线粒体：从细胞和分子角度看
Front Physiol. 2024 Jun 21;15:1406635. doi: 10.3389/fphys.2024.1406635. eCollection 2024.
2
The Initial COVID-19 Reliable Interactive DNA Methylation Markers and Biological Implications.初始新冠病毒可靠的交互式DNA甲基化标记及其生物学意义。
Biology (Basel). 2024 Apr 7;13(4):245. doi: 10.3390/biology13040245.
3
Choosing a cellular model to study SARS-CoV-2.选择用于研究 SARS-CoV-2 的细胞模型。

本文引用的文献

1
Characterization of SARS-CoV-2 and host entry factors distribution in a COVID-19 autopsy series.SARS-CoV-2的特征及新型冠状病毒肺炎尸检系列中宿主进入因子的分布
Commun Med (Lond). 2021 Aug 23;1:24. doi: 10.1038/s43856-021-00025-z. eCollection 2021.
2
SARS-CoV-2 Proteins Bind to Hemoglobin and Its Metabolites.SARS-CoV-2 蛋白与血红蛋白及其代谢物结合。
Int J Mol Sci. 2021 Aug 21;22(16):9035. doi: 10.3390/ijms22169035.
3
CD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV-2.CD209L/L-信号素和CD209/树突状细胞特异性细胞间黏附分子-3抓取非整合素作为严重急性呼吸综合征冠状病毒2的受体。
Front Cell Infect Microbiol. 2022 Oct 21;12:1003608. doi: 10.3389/fcimb.2022.1003608. eCollection 2022.
4
Gene Networks of Hyperglycemia, Diabetic Complications, and Human Proteins Targeted by SARS-CoV-2: What Is the Molecular Basis for Comorbidity?高血糖、糖尿病并发症与 SARS-CoV-2 作用的人类蛋白的基因网络：共病的分子基础是什么？
Int J Mol Sci. 2022 Jun 29;23(13):7247. doi: 10.3390/ijms23137247.
ACS Cent Sci. 2021 Jul 28;7(7):1156-1165. doi: 10.1021/acscentsci.0c01537. Epub 2021 Jun 30.
4
SARS-CoV-2 Causes Acute Kidney Injury by Directly Infecting Renal Tubules.严重急性呼吸综合征冠状病毒2型通过直接感染肾小管导致急性肾损伤。
Front Cell Dev Biol. 2021 May 31;9:664868. doi: 10.3389/fcell.2021.664868. eCollection 2021.
5
Functional landscape of SARS-CoV-2 cellular restriction.SARS-CoV-2 细胞限制的功能景观
Mol Cell. 2021 Jun 17;81(12):2656-2668.e8. doi: 10.1016/j.molcel.2021.04.008. Epub 2021 Apr 13.
6
Mechanisms of Coronavirus Nsp1-Mediated Control of Host and Viral Gene Expression.冠状病毒 Nsp1 介导的宿主和病毒基因表达调控的机制。
Cells. 2021 Feb 2;10(2):300. doi: 10.3390/cells10020300.
7
Kidney injury molecule-1 is a potential receptor for SARS-CoV-2.肾损伤分子 1 是 SARS-CoV-2 的潜在受体。
J Mol Cell Biol. 2021 Jul 6;13(3):185-196. doi: 10.1093/jmcb/mjab003.
8
AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells.AXL 是 SARS-CoV-2 的候选受体，能促进肺和支气管上皮细胞的感染。
Cell Res. 2021 Feb;31(2):126-140. doi: 10.1038/s41422-020-00460-y. Epub 2021 Jan 8.
9
Host mitochondrial transcriptome response to SARS-CoV-2 in multiple cell models and clinical samples.宿主线粒体转录组对多种细胞模型和临床样本中的 SARS-CoV-2 的反应。
Sci Rep. 2021 Jan 8;11(1):3. doi: 10.1038/s41598-020-79552-z.
10
Heparan sulfate assists SARS-CoV-2 in cell entry and can be targeted by approved drugs in vitro.硫酸乙酰肝素有助于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）进入细胞，并且在体外可被已批准的药物靶向作用。
Cell Discov. 2020 Nov 4;6(1):80. doi: 10.1038/s41421-020-00222-5.