Lodha Divya, Subramaniam Jamuna R
Center for Preclinical and Translational Medical Research (CPTMR), Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
J Neurosci Rural Pract. 2022 Jan 13;13(1):114-118. doi: 10.1055/s-0041-1742120. eCollection 2022 Jan.
The main aim of this study is to identify the deleterious effects of indiscriminately consumed high fructose on motor neurons that are critically affected in many neurological conditions causing movement disorders including paralysis. Neuroblastoma x mouse spinal cord motor neuron cell line (NSC-34) motor neuron cell lines were treated with high fructose and oxygen supplementation (18.8%) and assayed for cell proliferation/death, reactive oxygen species (ROS) generation, and oxidative stress response induction Mean and standard deviation, significance with and without high fructose (F)-5%, were estimated by -tests using GraphPad Prism ver. 8.2.1 F-5% along with O (18.8%) annihilates the cells (∼85%) by day10 and inhibits cell division as observed by the presence of multinucleated cells. Unexpectedly, 1 to 2% of cells that survived, differentiated and displayed progressive neurite extension. Though not healthy, they were viable up to 80 days. F-5% increased ROS levels (∼34%) not accompanied by concomitant enhanced expression of oxidative stress response regulator, the transcription factor, , or downstream effector, High fructose is extremely harmful to NSC-34 motor neuron cell line.
本研究的主要目的是确定不加区分地摄入高果糖对运动神经元的有害影响，在许多导致运动障碍（包括瘫痪）的神经系统疾病中，运动神经元会受到严重影响。 将神经母细胞瘤x小鼠脊髓运动神经元细胞系（NSC-34）运动神经元细胞系用高果糖和补充氧气（18.8%）处理，并检测细胞增殖/死亡、活性氧（ROS）生成和氧化应激反应诱导情况 使用GraphPad Prism 8.2.1版本通过t检验估计平均值和标准差，以及有无5%高果糖（F）时的显著性。 5%的F与O₂（18.8%）一起在第10天时使细胞凋亡（约85%），并通过多核细胞的存在观察到抑制细胞分裂。出乎意料的是，1%至2%存活的细胞分化并显示出渐进性的神经突延伸。虽然不健康，但它们可以存活长达80天。5%的F使ROS水平增加（约34%），但没有伴随氧化应激反应调节因子、转录因子或下游效应器的表达增强。 高果糖对NSC-34运动神经元细胞系极其有害。
