Torres Filho Ivo P, Torres Luciana N, Barraza David, Williams Charnae E, Hildreth Kim
Hemorrhage and Edema Control, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, USA.
Dose Response. 2022 Jan 29;20(1):15593258211073100. doi: 10.1177/15593258211073100. eCollection 2022 Jan-Mar.
Evaluating the impact of ionizing radiation on stored blood is relevant since blood banks are major assets in emergency conditions such as radiation incident/attack. This study aimed to fill our knowledge gap of combined radiation and storage effects on blood.
Blood collected from 16 anesthetized rats was anticoagulated, aliquoted into storage bags, and assigned to 8 groups using protocols combining storage (1-day vs 3-day 4C) plus irradiation (75 Gy vs 0 Gy - control). Bags were positioned inside an X-ray irradiator (MultiRad-350). Complete blood count, differential white blood cell count, biochemistry, and hemostasis were analyzed (≥7 bags/group).
Na, bicarbonate, glucose, and pH significantly reduced, while K, Cl, and lactate increased by storage. Coagulation measures were not significantly altered after radiation. White blood cell count and most cell types were numerically reduced after radiation, but changes were statistically significant only for monocytes. No significant alterations were noted in aggregation or rotational thromboelastometry parameters between irradiated and control.
Evaluating cellular/biochemical parameters aids in assessing stored blood adequacy after radiation. Data suggest that fresh or cold-stored blood can sustain up to 75 Gy without major critical parameter changes and may remain suitable for use in critically ill patients in military/civilian settings.
评估电离辐射对储存血液的影响具有重要意义，因为血库是辐射事故/袭击等紧急情况下的重要资产。本研究旨在填补我们在辐射与储存对血液综合影响方面的知识空白。
从16只麻醉大鼠采集血液，抗凝后分装到储存袋中，采用储存（1天与3天4℃）加照射（75 Gy与0 Gy - 对照）的方案分为8组。袋子放置在X射线辐照器（MultiRad - 350）内。分析全血细胞计数、白细胞分类计数、生化指标和止血指标（每组≥7个袋子）。
储存后钠、碳酸氢根、葡萄糖和pH显著降低，而钾、氯和乳酸升高。辐射后凝血指标无显著变化。辐射后白细胞计数和大多数细胞类型数量减少，但仅单核细胞的变化具有统计学意义。照射组与对照组之间的聚集或旋转血栓弹力图参数无显著改变。
评估细胞/生化参数有助于评估辐射后储存血液的充足性。数据表明，新鲜或冷藏血液在接受高达75 Gy的辐射后，主要关键参数无重大变化，可能仍适用于军事/民用环境中的重症患者。
