Finney P M, Bushell A C
J Immunol Methods. 1986 Jan 22;86(1):71-4. doi: 10.1016/0022-1759(86)90266-8.
A method is described which permits the assay of specific secretory immunoglobulin A (sIgA) antibodies produced by guinea pigs in response to ocular infection with the guinea pig inclusion conjunctivitis strain of Chlamydia psittaci (GPIC agent). The enzyme-linked immunosorbent assay (ELISA) developed was shown to be more sensitive and less subjective than the micro-immunofluorescence assay as a means of assaying specific antibody.
本文描述了一种方法,该方法可用于检测豚鼠因感染鹦鹉热衣原体豚鼠包涵体结膜炎菌株(GPIC病原体)而产生的特异性分泌型免疫球蛋白A(sIgA)抗体。作为一种检测特异性抗体的方法,所开发的酶联免疫吸附测定法(ELISA)比微量免疫荧光测定法更灵敏且主观性更低。