Levy N J, Muñoz A, McCormack W M
J Lab Clin Med. 1983 Dec;102(6):918-25.
An enzyme-linked immunosorbent assay (ELISA) was developed with use of a strain of Chlamydia trachomatis, lymphogranuloma venereum serotype 2. The ELISA reactions were monitored by absorbance at 492 nm in a spectrophotometer. A positive control method based on one serum dilution was used to assign ELISA titers to the sera. Sera that had been tested with use of complement fixation (CF) and microimmunofluorescence (MIF) tests were examined with ELISA. ELISA was more sensitive than the CF test and as sensitive as the MIF test. Serum pairs that had diagnostic titer rises in the other tests also had such rises in ELISA. ELISA is a simple, sensitive assay for detection of antibody to C. trachomatis.
利用一株沙眼衣原体性病淋巴肉芽肿血清型2开发了一种酶联免疫吸附测定（ELISA）。在分光光度计中通过492nm处的吸光度监测ELISA反应。使用基于一种血清稀释度的阳性对照方法来确定血清的ELISA滴度。用补体结合（CF）和微量免疫荧光（MIF）试验检测过的血清用ELISA进行检测。ELISA比CF试验更敏感，与MIF试验一样敏感。在其他试验中诊断滴度升高的血清对在ELISA中也有这种升高。ELISA是一种检测沙眼衣原体抗体的简单、灵敏方法。
