Szczotka-Bochniarz Anna, Karamon Jacek, Nowak Agnieszka, Porowski Marian, Karbowiak Paweł, Holeniewski Andrzej, Pejsak Zygmunt
Department of Swine Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland.
Department of Parasitology, National Veterinary Research Institute, 24-100 Puławy, Poland.
J Vet Res. 2021 Oct 26;65(4):425-430. doi: 10.2478/jvetres-2021-0057. eCollection 2021 Dec.
Diarrhoea in growing-finishing pigs is a common problem of commercial pig farms. Among many causative factors, porcine circovirus type 2 (PCV2) is one considered an important pathogen in modern pig production. The aim of the study was to verify if PCV2 was responsible for antibiotic non-responsive diarrhoea and wasting in pigs.
A total of 13 dead pigs aged between 12 and 15 weeks from three Polish farms with persistent herd symptoms suggestive of PCV2 infection were provided for evaluation. Sections of lymph nodes and intestines were analysed by hybridization (ISH) for PCV2 and histopathological examination. Faeces and intestinal scrapings were tested for and by real-time PCR and for parasitic infection by flotation and decantation.
ISH and histopathological examination showed that all pigs were PCV2 systemic disease negative. Swine dysentery was confirmed by real-time PCR on two farms, and proliferative enteropathy on one farm. In histological examinations, erosions of the caecal and colonic mucosa were found, together with cysts and trophozoites of . The protozoa were present in the intestinal lumen and mucosa. cysts were identified in faeces from all examined pigs.
These results suggest that monitoring of infections should be an additional measure of control and prevention of gastrointestinal tract disorders in modern swine husbandry.
生长育肥猪腹泻是商业养猪场的常见问题。在众多致病因素中,猪圆环病毒2型(PCV2)被认为是现代养猪生产中的一种重要病原体。本研究的目的是验证PCV2是否导致猪的抗生素无反应性腹泻和消瘦。
从三个有持续群体症状提示PCV2感染的波兰农场提供了13头12至15周龄的死猪进行评估。通过原位杂交(ISH)分析淋巴结和肠道切片中的PCV2,并进行组织病理学检查。通过实时PCR检测粪便和肠道刮片的 和 ,并通过浮选和倾析检测寄生虫感染。
ISH和组织病理学检查表明,所有猪均为PCV2系统性疾病阴性。通过实时PCR在两个农场确诊为猪痢疾,在一个农场确诊为增生性肠炎。在组织学检查中,发现盲肠和结肠黏膜糜烂,伴有 的囊肿和滋养体。原生动物存在于肠腔和黏膜中。在所有检查猪的粪便中均鉴定出 囊肿。
这些结果表明,监测 感染应成为现代养猪业控制和预防胃肠道疾病的一项额外措施。