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从丝状真菌中提取 DNA 的正交方案。

Orthogonal protocols for DNA extraction from filamentous fungi.

机构信息

Section for Ecology and Evolution, Department of Biology, University of Copenhagen, København, Denmark.

Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Ancon, Panama.

出版信息

STAR Protoc. 2022 Jan 24;3(1):101126. doi: 10.1016/j.xpro.2022.101126. eCollection 2022 Mar 18.

DOI:10.1016/j.xpro.2022.101126
PMID:35112085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8790498/
Abstract

There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021).

摘要

从真菌中提取 DNA 的方法很少。本文提供了四种从真菌中提取基因组 DNA 的互补方法。我们定量评估了提取效果,并比较了来自五个丝状真菌属的 8 个物种,包括担子菌和子囊菌。这些方法应该对从各种丝状真菌中提取 DNA 有用。有关该方案的使用和执行的完整详细信息,请参阅 Conlon 等人(2021 年)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8790498/a642cc44f7af/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8790498/049245c55105/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8790498/ea723a6b2875/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8790498/a642cc44f7af/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8790498/049245c55105/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8790498/ea723a6b2875/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8790498/a642cc44f7af/gr2.jpg

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本文引用的文献

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iScience. 2021 Jun 1;24(6):102680. doi: 10.1016/j.isci.2021.102680. eCollection 2021 Jun 25.
2
Evaluation of three methods for effective extraction of DNA from human hair.三种从人发中有效提取DNA方法的评估。
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jun 5;820(1):137-41. doi: 10.1016/j.jchromb.2004.11.028. Epub 2005 Apr 11.
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Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
优化从模拟血液培养标本中进行牛津纳米孔非靶向鸟枪法宏基因组测序的真菌DNA提取和纯化方法。
mSystems. 2025 Jun 17;10(6):e0116624. doi: 10.1128/msystems.01166-24. Epub 2025 Apr 8.
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Unveiling the Potential Role of Dhurrin in Sorghum During Infection by the Head Smut Pathogen f. sp. .揭示高粱醛在高粱丝黑穗病菌侵染过程中的潜在作用 。(注:原文中“f. sp.”后面似乎内容不完整)
Plants (Basel). 2025 Feb 28;14(5):740. doi: 10.3390/plants14050740.
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Volatile Organic Compounds of Diverse Origins and Their Changes Associated With Cultivar Decay in a Fungus-Farming Termite.不同来源的挥发性有机化合物及其与培养真菌的白蚁品种衰退相关的变化
Environ Microbiol. 2025 Feb;27(2):e70049. doi: 10.1111/1462-2920.70049.
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Non-ribosomal peptide synthase profiles remain structurally similar despite minimally shared features across fungus-farming termite microbiomes.尽管真菌养殖白蚁微生物群之间的共同特征极少,但非核糖体肽合成酶谱在结构上仍保持相似。
ISME Commun. 2024 Jul 11;4(1):ycae094. doi: 10.1093/ismeco/ycae094. eCollection 2024 Jan.
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Comparative genomics unravels a rich set of biosynthetic gene clusters with distinct evolutionary trajectories across fungal species (Termitomyces) farmed by termites.比较基因组学揭示了一系列丰富的生物合成基因簇,这些基因簇在白蚁养殖的真菌物种(Termitomyces)中具有不同的进化轨迹。
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J Nat Prod. 2024 Sep 27;87(9):2204-2215. doi: 10.1021/acs.jnatprod.4c00516. Epub 2024 Aug 16.
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