从丝状真菌中提取 DNA 的正交方案。
Orthogonal protocols for DNA extraction from filamentous fungi.
机构信息
Section for Ecology and Evolution, Department of Biology, University of Copenhagen, København, Denmark.
Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Ancon, Panama.
出版信息
STAR Protoc. 2022 Jan 24;3(1):101126. doi: 10.1016/j.xpro.2022.101126. eCollection 2022 Mar 18.
There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021).
从真菌中提取 DNA 的方法很少。本文提供了四种从真菌中提取基因组 DNA 的互补方法。我们定量评估了提取效果，并比较了来自五个丝状真菌属的 8 个物种，包括担子菌和子囊菌。这些方法应该对从各种丝状真菌中提取 DNA 有用。有关该方案的使用和执行的完整详细信息，请参阅 Conlon 等人（2021 年）。
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