Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Plant Signal Behav. 2022 Dec 31;17(1):2026614. doi: 10.1080/15592324.2022.2026614. Epub 2022 Feb 3.
Reproductive success of plants greatly depends on the proper timing of the floral transition, which is precisely controlled by a complex genetic network. (), a central floral repressor, is transcriptionally activated by the FRIGIDA (FRI) activator complex including FLC EXPRESSOR (FLX) and FLX-LIKE 4 (FLX4). C-TERMINAL DOMAIN PHOSPHATASE-LIKE 3 (CPL3) forms a protein complex with FLX and FLX4 to mediate the dephosphorylation of FLX4, thereby promoting expression to repress flowering in both winter and summer annuals. Here, we show that CPL2 acts redundantly with CPL3 to mediate activation and flowering time. Similar to CPL3, CPL2 inhibits the floral transition, and is required for basal expression in summer annuals and activation in winter annuals. CPL2 directly interacts with FLX which further bridges the interaction between CPL2 and FLX4. Our results suggest that CPL2 and CPL3 function redundantly in regulating expression to prevent precocious flowering.
植物的生殖成功在很大程度上取决于花发育转变的适时发生，而这一过程受到一个复杂的遗传网络的精确调控。（）作为一个中央花抑制子，其转录活性受到 FRIGIDA (FRI)激活复合物的调控，该复合物包括 FLC EXPRESSOR (FLX) 和 FLX-LIKE 4 (FLX4)。C-TERMINAL DOMAIN PHOSPHATASE-LIKE 3 (CPL3) 与 FLX 和 FLX4 形成蛋白复合物，介导 FLX4 的去磷酸化，从而促进表达，抑制冬性一年生和夏性一年生植物的开花。在这里，我们表明 CPL2 与 CPL3 冗余发挥作用以介导 的激活和开花时间。与 CPL3 相似，CPL2 抑制花发育转变，并需要在夏性一年生植物中维持基础 的表达和在冬性一年生植物中激活 。CPL2 直接与 FLX 相互作用，进一步桥接了 CPL2 和 FLX4 之间的相互作用。我们的结果表明，CPL2 和 CPL3 冗余发挥作用以调节 的表达，以防止早熟开花。
