Reduction of ROS-HIF1α-driven glycolysis by taurine alleviates infection.

Antibiotic-resistant strains of () frequently cause clinical mastitis in dairy cows resulting in enormous economic losses. The regulation of immunometabolism is a promising strategy for controlling this bacterial infection. To investigate whether taurine alleviates infection by the regulation of host glycolysis HIF1α, the murine mammary epithelial cell line (EpH4-Ev) and C57BL/6J mice were challenged with Our data indicate that HIF1α-driven glycolysis promotes inflammation and damage in response to the challenge. The activation of HIF1α is dependent on mTOR-mediated ROS production. These results were confirmed . Taurine, an intracellular metabolite present in most animal tissues, has been shown to effectively modulate HIF1α-triggered metabolic reprogramming and contributes to a reduction of inflammation, which reduces mammary tissue damage and prevents mammary gland dysfunction in -induced mastitis. These data provide a novel putative prophylactic and therapeutic strategy for amelioration of dairy cow mastitis and bacterial inflammation.