Department of Biology, Science Faculty, Ataturk University, Erzurum, Turkey.
Köprüköy Anatolian High School, Erzurum, Turkey.
Arch Microbiol. 2022 Feb 3;204(3):159. doi: 10.1007/s00203-022-02765-5.
Extensive use of pesticides has led to the contamination of ecosystem. Therefore, it is important to isolate potential new pesticide-degrading bacteria. For the biodegradation of α-endosulfan and α-cypermethrin, a new bacterium was isolated from the body microflora of grasshopper (Poecilimon tauricola). Based on biochemical, morphological, and 16S rRNA sequence analysis, the isolated strain B7 was identified as Acinetobacter schindleri. This bacterial strain was screened for its α-cypermethrin and α-endosulfan degrading potential with minimal salt medium (MSM) and non-sulfur medium (NSM), respectively. When glucose was added to non-sulfur medium containing α-endosulfan (100 mg/L) and minimal salt medium containing α-cypermethrin (100 mg/L), both pesticide degradation and bacterial growth were increased. Acinetobacter schindleri B7 was able to degrade 67.31% of α-endosulfan and 68.4% of α-cypermethrin within 10 days. The degradation products of pesticides were determined by HPLC. As a result, A. schindleri, a Gram-negative bacterium, can inevitably be used in the biological treatment of environments exposed to pesticides.
大量使用农药导致了生态系统的污染。因此，分离潜在的新型农药降解菌非常重要。为了实现 α-硫丹和 α-氯氰菊酯的生物降解，从蝗虫（Poecilimon tauricola）体微生物群中分离到一种新的细菌。基于生化、形态和 16S rRNA 序列分析，分离出的菌株 B7 被鉴定为不动杆菌属（Acinetobacter）。使用最小盐培养基（MSM）和非硫培养基（NSM）分别筛选该细菌对 α-氯氰菊酯和 α-硫丹的降解潜力。当向含有 α-硫丹（100mg/L）的非硫培养基和含有 α-氯氰菊酯（100mg/L）的最小盐培养基中添加葡萄糖时，农药降解和细菌生长都得到了提高。不动杆菌属（Acinetobacter）B7 能够在 10 天内降解 67.31%的 α-硫丹和 68.4%的 α-氯氰菊酯。通过 HPLC 确定了农药的降解产物。结果表明，革兰氏阴性菌不动杆菌属（Acinetobacter）可用于生物处理接触农药的环境。
