Neu J, Ozaki C K, Angelides K J
Pediatr Res. 1986 Jan;20(1):79-82. doi: 10.1203/00006450-198601000-00022.
The normal biophysical properties of the maturing mammalian enterocyte membrane are poorly understood. While the effects of glucocorticoids on maturation of intestinal enzyme function has been intensively investigated, their effects on membrane biophysical properties are not known. We used 1,6-diphenyl-1,3,5-hexatriene as a probe in fluorescence anisotropy studies to determine the fluidity of rat brush border membrane. Maturational changes and the effects of glucocorticoids administered antenatally or postnatally were determined. Fluorescence anisotropy values for 1,6-diphenyl-1,3,5-hexatriene in mature brush border membranes were higher than those values in membranes obtained from younger animals reflecting a less fluid membrane. Glucocorticoids administered to suckling rats increased the anisotropy values of 1,6-diphenyl-1,3,5-hexatriene in the membranes compared to saline-administered littermates. The anisotropy of intestinal brush border membrane was also increased in fetal rats whose mothers received dexamethasone. These alterations may relate to protein-binding properties and permeability characteristics of the enterocyte membrane.
成熟哺乳动物肠上皮细胞膜的正常生物物理特性目前仍知之甚少。虽然糖皮质激素对肠道酶功能成熟的影响已得到深入研究，但其对膜生物物理特性的影响尚不清楚。我们使用1,6 - 二苯基 - 1,3,5 - 己三烯作为荧光各向异性研究中的探针，以确定大鼠刷状缘膜的流动性。研究了成熟过程中的变化以及产前或产后给予糖皮质激素的影响。成熟刷状缘膜中1,6 - 二苯基 - 1,3,5 - 己三烯的荧光各向异性值高于幼年动物膜中的值，这反映出膜的流动性较低。与给予生理盐水的同窝仔鼠相比，给乳鼠注射糖皮质激素会增加膜中1,6 - 二苯基 - 1,3,5 - 己三烯的各向异性值。在其母亲接受地塞米松的胎鼠中，肠刷状缘膜的各向异性也增加了。这些改变可能与肠上皮细胞膜的蛋白质结合特性和通透性特征有关。
