Nomoto K, Mayumi H, Himeno K, Watanabe Y, Mitani M, Tokuda N
Transplantation. 1986 Feb;41(2):209-13. doi: 10.1097/00007890-198602000-00015.
Skin allograft survival and immune responses against allogeneic antigens homologous to skin grafts were observed in BALB/c Cr Slc (BALB) mice (H-2d) thymectomized at 1 day after birth and grafted with skin from major histocompatibility complex (MHC)-incompatible, fully allogeneic C3H/HeN (C3H) (H-2k) or MHC-compatible allogeneic DBA/2 Cr Slc (DBA) mice (H-2d), at 14 weeks of age. In neonatally thymectomized (NTx) BALB mice, survival of C3H skin grafts was not prolonged at all, but survival of DBA skin grafts was prolonged significantly, although the survival periods of DBA skin grafts were very different among individual recipients. In NTx recipients grafted with C3H skin, delayed foot-pad reaction (DFR) was not reduced, but cytotoxic lymphocyte (CTL) activity and cytotoxic antibody (CTAb) production were appreciably depressed. CTL and CTAb were reduced profoundly and consistently in all NTx mice grafted with DBA skin, while DFR was reduced to various degrees in each. The degrees of depression of DFR in these NTx mice correlated well with the prolongation of DBA skin survival, although the sample number was small. The rejection of skin allografts appears to be attributable largely to a T cell subset, the function of which can be expressed as DFR. Thymus dependency in the ontogenic development is low as compared with other T cell subsets.
观察出生后1天胸腺切除的BALB/c Cr Slc（BALB）小鼠（H-2d），在14周龄时移植来自主要组织相容性复合体（MHC）不相容的完全同种异体C3H/HeN（C3H）（H-2k）或MHC相容的同种异体DBA/2 Cr Slc（DBA）小鼠（H-2d）的皮肤后，同种异体皮肤移植的存活情况以及针对与皮肤移植同源的同种异体抗原的免疫反应。在新生胸腺切除（NTx）的BALB小鼠中，C3H皮肤移植的存活时间完全没有延长，但DBA皮肤移植的存活时间显著延长，尽管不同个体受体中DBA皮肤移植的存活期差异很大。在移植C3H皮肤的NTx受体中，迟发型足垫反应（DFR）没有降低，但细胞毒性淋巴细胞（CTL）活性和细胞毒性抗体（CTAb）产生明显受到抑制。在所有移植DBA皮肤的NTx小鼠中，CTL和CTAb均显著且持续降低，而DFR在每只小鼠中都有不同程度的降低。尽管样本数量较少，但这些NTx小鼠中DFR的降低程度与DBA皮肤存活时间的延长密切相关。皮肤同种异体移植的排斥反应似乎主要归因于一个T细胞亚群，其功能可以表现为DFR。与其他T细胞亚群相比，其在个体发育中的胸腺依赖性较低。
