Mitsuyama M, Watanabe Y, Sano M, Amako K, Nomoto K
Department of Bacteriology, Niigata University School of Medicine, Japan.
Med Microbiol Immunol. 1988;177(4):207-17. doi: 10.1007/BF00211220.
Neonatally thymectomized (NTx) mice, sham-operated control mice and congenitally athymic nude mice were immunized with viable Listeria monocytogenes and their spleen cells examined for the capacity to transfer both delayed footpad reaction and protection against challenge at the site of local transfer. Cells from immune NTx mice conferred significant degrees of delayed footpad reaction and protection comparable to sham mice, while cells from immune nude (nu/nu) mice did not. This ability was completely eliminated by the treatment of cells with anti-Thy1, anti-Lyt1 or anti-L3T4 antibody plus complement but not with anti-Lyt2 antibody plus complement. These results indicated that NTx mice can normally mount the immunity to L. monocytogenes by generating Lyt1+2-, L3T4+ T cells. Immune competence of NTx mice and thymus dependency of various immune responses are discussed.
对新生期切除胸腺的（NTx）小鼠、假手术对照小鼠和先天性无胸腺裸鼠用活的单核细胞增生李斯特菌进行免疫，并检测它们的脾细胞传递延迟性足垫反应以及在局部转移部位抵御攻击的能力。来自免疫NTx小鼠的细胞产生了与假手术小鼠相当程度的显著延迟性足垫反应和保护作用，而来自免疫裸鼠（nu/nu）的细胞则没有。用抗Thy1、抗Lyt1或抗L3T4抗体加补体处理细胞可完全消除这种能力，但用抗Lyt2抗体加补体处理则不会。这些结果表明，NTx小鼠能够通过产生Lyt1 + 2 -、L3T4 + T细胞正常地对单核细胞增生李斯特菌产生免疫。讨论了NTx小鼠的免疫能力以及各种免疫反应的胸腺依赖性。
