宫颈癌高表达长链非编码RNA 1促进甲状腺乳头状癌细胞增殖和侵袭。
Cervical carcinoma high-expressed long non-coding RNA 1 promotes papillary thyroid carcinoma cell proliferation and invasion.
作者信息
Shi Ping, Liu Yan, Zhang Mingzeng, Yang Jianwang, Jing Shanghua, Yang Dongqiang, Liu Fei, Wu Yanzhao, Shi Huijing, Geng Cuizhi
机构信息
Department of Otolaryngology Head and Neck Surgery, Hebei Medical University Fourth Affiliated Hospital, Shijiazhuang, China.
Department of Hematology, Hebei Medical University Fourth Affiliated Hospital, Shijiazhuang, China.
出版信息
Transl Cancer Res. 2021 Sep;10(9):4158-4168. doi: 10.21037/tcr-21-1502.
BACKGROUND
Studies have shown that cervical carcinoma high-expressed long non-coding RNA 1 () may promote tumor development by regulating tumor migration and invasion in a variety of cancers; yet, the role of in papillary thyroid carcinoma (PTC) remains unclear. The purpose of this study was to explore the mechanism of in PTC.
METHODS
The expression of in 51 PTC carcinoma tissues and normal adjacent tissues was measured using real-time quantitative polymerase chain reaction (RT-qPCR). Cell Counting Kit-8 (CCK8), plate cloning assay, transwell assay, and flow cytometry were used to analyze the effect of on PTC cell proliferation, invasion, and apoptosis .
RESULTS
A higher expression of was found in PTC tissues than in adjacent tissues. High expression of was positively correlated with the number of tumors, extra-glandular invasion, and tumor stage. In addition, the down-regulation of reduced the proliferation and invasion of PTC cell lines and promoted cell apoptosis, while its up-regulation caused the opposite effect. These effects were regulated via the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway.
CONCLUSIONS
The is closely related to PTC progression and may be used as a potential biomarker for early diagnosis and treatment of PTC.
背景
研究表明，宫颈癌高表达长链非编码RNA 1（）可能通过调节多种癌症中的肿瘤迁移和侵袭来促进肿瘤发展；然而，其在甲状腺乳头状癌（PTC）中的作用仍不清楚。本研究旨在探讨其在PTC中的作用机制。
方法
采用实时定量聚合酶链反应（RT-qPCR）检测51例PTC癌组织及癌旁正常组织中该基因的表达。使用细胞计数试剂盒-8（CCK8）、平板克隆试验、Transwell试验和流式细胞术分析其对PTC细胞增殖、侵袭和凋亡的影响。
结果
PTC组织中该基因的表达高于癌旁组织。该基因的高表达与肿瘤数量、腺外侵犯及肿瘤分期呈正相关。此外，该基因表达下调可降低PTC细胞系的增殖和侵袭能力，并促进细胞凋亡，而其表达上调则产生相反的效果。这些作用是通过细胞外信号调节激酶/丝裂原活化蛋白激酶（ERK/MAPK）途径介导的。
结论
该基因与PTC进展密切相关，可能作为PTC早期诊断和治疗的潜在生物标志物。
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