Department of Endocrinology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China.
Department of Nuclear Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China.
Cancer Biomark. 2018;22(2):217-226. doi: 10.3233/CBM-170777.
To investigate the expression and role of long non-coding RNA (lncRNA) small nucleolar RNA host gene 12 (SNHG12) in papillary thyroid carcinoma (PTC).
The relative expression levels of lncRNA SNHG12 (hereinafter referred to as SNHG12) in 42 pairs of PTC tissues and para-carcinoma tissues were detected via quantitative reverse transcription polymerase chain reaction (qRT-PCR). SNHG12 specific interference sequences were designed and synthesized. The relative expression level and transfection efficiency of SNHG12 in PTC cells were detected via qRT-PCR. After the interference in SNHG12 expression, the change in cell proliferation capacity was detected via methyl thiazolyl tetrazolium (MTT) assay, the change in cell cycle distribution was detected via flow cytometry, the changes in cell migration and invasion capacities were detected via Transwell assay and wound healing assay, and the changes in expressions of molecular markers of Wnt/β-catenin pathway were detected via Western blotting. The pulmonary metastasis model of nude mice was established, and the changes in migration and invasion capacities of tumor cells were studied via the in-vivo experiment after the interference in SNHG12 expression.
The results of qRT-PCR showed that the SNHG12 expression was up-regulated in 30 pairs of PTC tissues and cells. The results of MTT assay showed that the cell proliferation capacity was inhibited after the interference in SNHG12. The results of flow cytometry showed that the cell cycle progression was blocked in G1-G0 phase after the knockdown of SNHG12 expression. The results of Transwell assay and Western blotting showed that the interference in SNHG12 could inhibit the invasion and metastasis capacities of tumor cells through influencing the Wnt/β-catenin signaling pathway. Metastatic tumor model of nude mice showed that SNHG12 could affect the invasion and metastasis of tumor cells in vivo.
The SNHG12 expression is relatively high in PTC tissues and cells. In-vivo/in-vitro experiments prove that SNHG12 can promote the proliferation and metastasis of PTC cells through influencing the Wnt/β-catenin signaling pathway.
研究长链非编码 RNA（lncRNA）小核仁 RNA 宿主基因 12（SNHG12）在甲状腺乳头状癌（PTC）中的表达和作用。
通过实时荧光定量聚合酶链反应（qRT-PCR）检测 42 对 PTC 组织和癌旁组织中 lncRNA SNHG12（以下简称 SNHG12）的相对表达水平。设计并合成 SNHG12 特异性干扰序列。通过 qRT-PCR 检测 PTC 细胞中 SNHG12 的相对表达水平和转染效率。干扰 SNHG12 表达后，通过噻唑蓝（MTT）比色法检测细胞增殖能力的变化，通过流式细胞术检测细胞周期分布的变化，通过 Transwell 实验和划痕愈合实验检测细胞迁移和侵袭能力的变化，通过 Western blot 检测 Wnt/β-catenin 通路分子标志物表达的变化。建立裸鼠肺转移模型，通过体内实验研究干扰 SNHG12 表达后肿瘤细胞迁移和侵袭能力的变化。
qRT-PCR 结果显示，30 对 PTC 组织和细胞中 SNHG12 表达上调。MTT 检测结果显示，干扰 SNHG12 后细胞增殖能力受到抑制。流式细胞术结果显示，下调 SNHG12 表达后细胞周期阻滞于 G1-G0 期。Transwell 实验和 Western blot 结果显示，干扰 SNHG12 可通过影响 Wnt/β-catenin 信号通路抑制肿瘤细胞的侵袭和转移能力。裸鼠转移瘤模型显示，SNHG12 可影响肿瘤细胞在体内的侵袭和转移。
SNHG12 在 PTC 组织和细胞中表达较高。体内/体外实验证明，SNHG12 可通过影响 Wnt/β-catenin 信号通路促进 PTC 细胞的增殖和转移。
