Yan Hui-Xian, Du Jin, Fu Jing, Huang Wei, Jia Li-Meng, Ping Pang, Zhao Ling, Song Ye-Qiong, Jia Xiao-Meng, Dou Jing-Tao, Mu Yi-Ming, Wang Fu-Lin, Tian Wen, Lyu Zhao-Hui
Department and Key Laboratory of Endocrinology and Metabolism, PLA General Hospital, Beijing 100853, China.
Department of Endocrinology, Beijing Haidian Hospital, Beijing Haidian Section of Peking University Third Hospital, Beijing 100080, China.
Transl Cancer Res. 2019 Apr;8(2):439-451. doi: 10.21037/tcr.2019.02.12.
Long noncoding RNAs (lncRNAs) can regulate the expression of genes at almost every level. The altered expression of lncRNAs was observed in many kinds of cancers. Until recently, few studies have focused on the function of lncRNAs in the context of papillary thyroid carcinoma (PTC).
In the current study, we collected seven PTC and nodular goiter tissue samples and explored mRNA and lncRNA expression patterns in these samples by microarray.
We observed aberrant expression of 94 lncRNAs and 99 mRNAs in the seven PTC samples as compared to the nodular goiter tissue [fold change (FC) ≥2.0; P<0.01]. To confirm these microarray results, quantitative polymerase chain reaction (q-PCR) was performed to assess the expression of three randomly selected differentially expressed mRNAs and lncRNAs, confirming our microarray findings significantly. We then performed gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analyses to systematically characterize the twelve significantly differential genes. A co-expression analysis revealed that the lncRNAs n382996, n342483, and n409114 were closely related to the regulation of MT1G, MT1H, and MT1F.
In the present study a string of novel lncRNAs associated with PTC were identified. Further study of these lncRNAs should be performed to identify novel target molecules which may improve diagnosis and treatment of PTC.
长链非编码RNA（lncRNAs）几乎可以在各个水平上调控基因表达。在多种癌症中都观察到lncRNAs表达的改变。直到最近，很少有研究关注lncRNAs在甲状腺乳头状癌（PTC）中的功能。
在本研究中，我们收集了7例PTC和结节性甲状腺肿组织样本，并通过微阵列技术探索了这些样本中的mRNA和lncRNA表达模式。
与结节性甲状腺肿组织相比，我们在7例PTC样本中观察到94种lncRNAs和99种mRNAs的异常表达[倍数变化（FC）≥2.0；P<0.01]。为了证实这些微阵列结果，进行了定量聚合酶链反应（q-PCR）以评估随机选择的3种差异表达的mRNAs和lncRNAs的表达，显著证实了我们的微阵列发现。然后我们进行了基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析，以系统地表征这12个显著差异基因。共表达分析显示lncRNAs n382996、n342483和n409114与MT1G、MT1H和MT1F的调控密切相关。
在本研究中，鉴定出了一系列与PTC相关的新型lncRNAs。应对这些lncRNAs进行进一步研究，以鉴定可能改善PTC诊断和治疗的新型靶分子。
